Inflammatory activation and myelin alterations in the hippocampus of post-traumatic stress disorder rats
10.19405/j.cnki.issn1000-1492.2024.12.010
- Author:
Luodong Yang
1
;
Yan Shi
2
;
Ziwei Zhang
2
;
Bin Li
2
;
Guiqing Zhang
3
Author Information
1. Dept of Clinical Psychology , The First Afiliated Hospital of Shihezi University , Shihezi 832000 ; Dept of Clinical Medicine , Shihezi University Medical School , Shihezi 832000
2. Dept of Clinical Medicine , Shihezi University Medical School , Shihezi 832000
3. Dept of Clinical Psychology , The First Afiliated Hospital of Shihezi University , Shihezi 832000 ;
- Publication Type:Journal Article
- Keywords:
post-traumatic stress disorder;
hippocampus;
NLRP3;
myelin;
neurons;
microglia
- From:
Acta Universitatis Medicinalis Anhui
2024;59(12):2135-2141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the alterations in hippocampal neurons,nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3) inflammasome,microglia,and myelin in rats with post-traumatic stress disorder(PTSD).
Methods:A PTSD rat model was established using the single prolonged stress(SPS) paradigm.Anxiety and cognitive functions were evaluated through the open field test,elevated plus maze,and Morris water maze.Histopathological changes in hippocampal neurons were assessed using hematoxylin and eosin(HE)staining.The expression levels of NLRP3 and iba-1(a microglial marker) in the hippocampus were examined using immunofluorescence staining.Immunohistochemical staining and Luxol Fast Blue staining were performed to investigate alterations in hippocampal myelin.
Results:Hippocampal neurons in PTSD rats exhibited damage,with increased activation of the NLRP3 inflammasome and microglia,and elevated myelin content.
Conclusion:The pathogenesis of PTSD may be associated with hippocampal neuronal damage,inflammatory responses,and changes in myelin.
- Full text:2025022715493234892创伤后应激障碍大鼠海马区炎症激活及髓鞘改变_杨罗东.pdf
