Whole-body dynamic 18 F-FDG PET/CT Patlak imaging to evaluate the radiosensitization early efficacy in rat glioma
10.19405/j.cnki.issn1000-1492.2022.07.014
- Author:
Ke Cai
1
;
Qingqing Zhang
1
;
Wenjing Yu
1
;
Yangyang Xue
1
;
Hui Wang
1
;
Huiqin Xu
1
Author Information
1. Dept of Nuclear Medicine，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
whole-body dynamic PET / CT;
patlak;
;
glioma;
;
oleanolic acid;
Ki-67;
GLUT-1
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1088-1093
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To investigate the value of whole-body dynamic18F-flurodeoxygluse positron emission tomography with computed tomography(18F-FDG PET/CT) Patlak imaging for evaluating the radiosensitization early efficacy in C6 rat glioma.
Methods:Twenty-four C6 rat glioma models were established and randomly divided into control group, Oleanolic acid(OA) group, radiotherapy group and OA+radiotherapy group, with 6 rats in each group. 75 min whole-body dynamic18F-FDG PET/CT Patlak imaging was performed before and 48 h after treatment. The left ventricular time-activity curve(TAC) of tumor-bearing rats was used as input function for Patlak analysis to obtain Ki images. The last frame of the dynamic PET scan was selected for static analysis, and the changes of maximum standardized uptake value(SUVmax), Ki values and tumor volume were observed, and the survival time of tumor-bearing rats was recorded. Then, the tumor samples were dissected for Ki-67 and glucose transporter-1(GLUT-1) immunohistochemical staining.
Results:Before treatment, there were no significant differences in SUVmaxand Ki values among the 4 groups(F=0.909, 0.858,P>0.05). 48 h after treatment, SUVmaxand Ki values of control group and OA group increased significantly(t=-10.697,-17.516,P<0.01;t=-19.321,-9.887,P<0.01), SUVmaxand Ki values of radiotherapy group and OA+radiotherapy group decreased(t=5.629, 6.916,P<0.01;t=7.241, 10.490,P<0.01), there was no significant difference in SUVmaxbetween radiotherapy group and OA+radiotherapy group 48 h after treatment(t=1.233,P=0.246), while the Ki values in OA+radiotherapy group was significantly lower than that in radiotherapy group, the difference was statistically significant(t=3.051,P<0.05). The immunohistochemistry staining results suggested that the expression of Ki-67 and GLUT-1 in OA+radiotherapy group was lower than that in the other 3 groups(F=63.887, 40.720,P<0.05). In addition, Ki values showed positive correlations with the expression of Ki-67 and GLUT-1(r=0.931, 0.936,P<0.01). Kaplan-Meier analysis showed that the survival time of tumor-bearing rat in OA+radiotherapy group was longer than that in the other three groups, and the difference was statistically significant(χ2=19.817,P<0.01).
Conclusion:OA has radiosensitization effect on C6 rat glioma. Whole-body dynamic18F-FDG PET/CT Patlak multi-parameter imaging can evaluate the radiosensitization early efficacy in C6 rat glioma, which is more sensitive than PET/CT static imaging.
- Full text:2025022708542795432全身动态~(18)F-FD...大鼠胶质瘤放疗增敏早期疗效_蔡可.pdf
