A Fitting Method for Photoacoustic Pump-probe Imaging Based on Phase Correction
10.16476/j.pibb.2024.0378
- VernacularTitle:一种基于相位矫正的光声泵浦成像拟合方法
- Author:
Zhuo-Jun XIE
1
;
Hong-Wen ZHONG
1
;
Run-Xiang LIU
1
;
Bo WANG
1
;
Ping XUE
2
;
Bin HE
2
Author Information
1. Department of Biomedical Engineering, School of Basic Medical Science, Central South University, Changsha 410083, China
2. State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China
- Publication Type:Journal Article
- Keywords:
photoacoustic pump-probe imaging;
lifetime fitting;
phase correction;
oxygen partial pressure
- From:
Progress in Biochemistry and Biophysics
2025;52(2):525-532
- CountryChina
- Language:Chinese
-
Abstract:
ObjectivePhotoacoustic pump-probe imaging can effectively eliminate the interference of blood background signal in traditional photoacoustic imaging, and realize the imaging of weak phosphorescence molecules and their triplet lifetimes in deep tissues. However, background differential noise in photoacoustic pump-probe imaging often leads to large fitting results of phosphorescent molecule concentration and triplet lifetime. Therefore, this paper proposes a novel triplet lifetime fitting method for photoacoustic pump-probe imaging. By extracting the phase of the triplet differential signal and the background noise, the fitting bias caused by the background noise can be effectively corrected. MethodsThe advantages and feasibility of the proposed algorithm are verified by numerical simulation, phantom and in vivo experiments, respectively. ResultsIn the numerical simulation, under the condition of noise intensity being 10% of the signal amplitude, the new method can optimize the fitting deviation from 48.5% to about 5%, and has a higher exclusion coefficient (0.88>0.79), which greatly improves the fitting accuracy. The high specificity imaging ability of photoacoustic pump imaging for phosphorescent molecules has been demonstrated by phantom experiments. In vivo experiments have verified the feasibility of the new fitting method proposed in this paper for fitting phosphoometric lifetime to monitor oxygen partial pressure content during photodynamic therapy of tumors in nude mice. ConclusionThis work will play an important role in promoting the application of photoacoustic pump-probe imaging in biomedicine.
