Study on the expression and therapeutic effect of miR-34a in mice with allergic rhinitis
10.19405/j.cnki.issn1000-1492.2022.06.014
- Author:
Weiwei Xie
1
;
Xiulian Lin
1
;
Qi Long
1
;
Guang Meng
1
;
Shuo Liu
1
Author Information
1. Dept of Otorhinolaryngology Head and Neck Surgery,Afiliated to Xiangya Medical College , Central South University, Haikou Hospital , Haikou 570208
- Publication Type:Journal Article
- Keywords:
allergic rhinitis;
miR-34a;
inhibitors;
inflammatory factors;
T cells
- From:
Acta Universitatis Medicinalis Anhui
2022;57(6):920-925
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and therapeutic effects of miR-34a in mice with allergic rhinitis.
Methods:10 mice were selected as blank control group, and 30 mice were used to construct mouse allergic rhinitis model with ovalbumin. They were randomly divided into model group, miR-34a inhibitor group and negative control group(NC group) with 10 cases each. 100 μl of physiological saline, 100 μl of negative transfection plasmid, and 100 μl of miR-34a inhibitor carrier mixed solution were injected into the tail vein respectively. The behavioral changes of mice after 1 week of intervention were observed; ELISA was used to detect the level of ovalbumin-specific IgE in mouse serum; qRT-PCR was used to detect interleukin(IL)-6, IL-8, IL-10, and IL-35, the relative expression of matrix metalloproteinase(MMP)-2, MMP-9 and vascular cell adhesion molecule(VCAM)-1 mRNA. The content of CD3+, CD4+, CD8+T cells in peripheral blood was detected by flow cytometry.
Results:The serum level of miR-34a mRNA in model mice was significantly higher than that in the blank group(P<0.05). Compared with the negative control group, the total behavioral score of mice after miR-34a inhibitor intervention was significantly reduced(P<0.05). The concentration of ovalbumin-specific IgE was significantly reduced(P<0.05), while IL-6, IL-8, IL-10, MMP-2, MMP-9 and VCAM-1 mRNA were significantly reduced(P<0.05), IL-35 increased significantly(P<0.05), and CD3+, CD4+/CD8+increased significantly(P<0.05). Compared with the blank control group, the levels of IL-2 and IFN-γ in the model group decreased(P<0.05); compared with the model group, the levels of IL-2 and IFN-γ in the miR-34a inhibitor group and NC group increased(P<0.05); those indexes of miR-34a inhibitor group and NC group were compared,and there were statistically significant differences(P<0.05); there was no difference between the model group and NC group.
Conclusion:miR-34a is highly expressed in mice with allergic rhinitis. Inhibition of miR-34a expression can significantly reduce the symptoms and inflammation of model mice, and improve immune function.
- Full text:2025022111220926386miR-34a在过敏性鼻炎小鼠中表达及治疗的作用研究_谢伟伟.pdf
