Study on the high expression of angiopoietin 1 in plasma of hyperhomocysteinemia rats and its protective effect on endothelial cells
10.19405/j.cnki.issn1000-1492.2022.05.002
- Author:
Tongtong Shen
1
;
Limin Zhou
1
;
Shuangshuang Dong
1
;
Xinxin Wang
1
;
Xiaohong Xu
1
;
Yu Liu
1
;
Fan Zheng
2
;
Shaobo Ma
2
;
Bing Shen
2
Author Information
1. Dept of Cardiology, The Afiliated Chuzhou Hospital of Anhui Medical University ( The First People ′s Hospital of Chuzhou) , Chuzhou 239000
2. Dept of Physiology, School of Basic Medical Science , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
homocysteine;
angiopoietin 1;
vascular endothelial cells;
label⁃free protein spectrum;
migration;
prolif⁃ eration
- From:
Acta Universitatis Medicinalis Anhui
2022;57(5):679-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of plasma protein expression profile in hyperhomocysteinemia rats and the protective effect of highly expressed angiopoietin 1 in plasma on endothelial cells.
Methods:The hyperhomocysteinemia animal model was established. The difference in plasma protein content was analyzed by label-free protein spectroscopy. The effects of homocysteine and angiopoietin 1 on endothelial cell migration and proliferation were detected by wound healing and CCK-8 proliferation assay.
Results:The results of protein profiling showed that 5 proteins were significantly up-regulated and 17 proteins were significantly down-regulated in the plasma of hyperhomocysteinemia rats, among which angiopoietin 1 was significantly up-regulated. In endothelial cells in the superior mesenteric artery of rats, treatment with 30 or 50 μmol/L homocysteine for 24 h significantly inhibited the migration and proliferation. Angiopoietin 1(600 ng/ml) significantly reduced the migration and proliferation of endothelial cells inhibited by 30 μmol/L homocysteine, but had no significant effect on the migration and proliferation of endothelial cells inhibited by 50 μmol/L homocysteine.
Conclusion:Hyperhomocysteinemia can significantly affect the protein expression profile in plasma. Angiopoietin 1 in plasma can compensate for the damage of vascular endothelial migration and proliferation function caused by homocysteine in a certain concentration range.
- Full text:2025021909313339685血管生成素1在高同型半胱氨...表达及对内皮细胞的保护作用_沈童童.pdf
