Study of miR-1285 on the proliferation, apoptosis and mechanism of chronic myeloid leukemia K562 cells through YAP
10.19405/j.cnki.issn1000-1492.2022.04.012
- Author:
Hongxia Zhang
1
;
Guangsheng Wu
2
Author Information
1. Dept of Hematology，The First Affiliated Hospital of Shihezi University Medical College，Shihezi 832000
2. Dept of Hematology，The First Affiliated Hospital of Shihezi University Medical College，Shihezi 832000Dept of Hematology，The First Affiliated Hospital of Shihezi University Medical College，Shihezi 832000
- Publication Type:Journal Article
- Keywords:
miR-1285;
YAP;
proliferation;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):569-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the miR-1285 through Yes-associated protein 1(YAP1) on the proliferation, apoptosis and mechanism of chronic myelogenous leukemia K562 cells.
Methods:The plasmid of miR-1285 was constructed to make K562 cells overexpress miR-1285 and knock down miR-1285. They were divided into four groups: miR-1285 mimics, mimics control, miR-1285 inhibitor, and inhibitor control; qRT-PCR was used to quantitatively analyze the expression of miR-1285 of these four groups. The expression level of miR-1285 in the group was used to verify the transfection efficiency. The apoptosis and proliferation of the four groups were detected by CCK-8 and Annexin V-FITC; the changes of YAP and its downstream molecule epidermal growth factor receptor(EGFR) were detected by Western blot, and apoptosis related molecules BAX, Bcl-2 protein expression was detected.
Results:After knock down miR-1285, it can promote the proliferation of K562 cells and inhibit apoptosis. The expression of YAP increased, the expression of downstream molecule EGFR increased correspondingly, the expression of apoptosis-related molecule BAX decreased, and the expression of Bcl-2 increased; After expressing miR-1285, K562 cell proliferation decreased, apoptosis increased, YAP expression decreased, downstream molecule P-EGFR expression decreased, apoptosis molecule BAX expression increased, and Bcl-2 expression decreased.
Conclusion:miR-1285 can inhibit the proliferation and promote apoptosis of K562 cells of chronic myelogenous leukemia by inhibiting the expression of YAP. It is expected to become a targeted molecule for the treatment of chronic myelogenous leukemia.
- Full text:2025021809302725928miR-1285通过YAP...殖、凋亡的影响及其作用机制_张红霞.pdf
