Cx43 regulates NLRP3 inflammasome and participates in α1-AR activation induced cardiac acute sympathetic stress
10.19405/j.cnki.issn1000-1492.2022.04.006
- Author:
Wenbo Wang
1
;
Yi Rong
2
;
Ling Chen
1
;
Xinzhi Li
2
;
Junqiang Si
2
;
Li Wang
1
;
Ketao Ma
2
Author Information
1. Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Diseases，School of Medicine， Shihezi University，Shihezi 832000; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases，Shihezi 832000; Dept of Cardiology，First Affiliated Hospital，School of Medicine， Shihezi University，Shihezi 832000;
2. Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Diseases，School of Medicine， Shihezi University，Shihezi 832000; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases，Shihezi 832000; Dept of Physiology，School of Medicine，Shihezi University，Shihezi 832000
- Publication Type:Journal Article
- Keywords:
connexin 43;
NLRP3 inflammasome;
phenylephrine;
myocardial cells;
acute sympathetic stress
- From:
Acta Universitatis Medicinalis Anhui
2022;57(4):534-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of connexin 43 ( Cx43) in acute sympathetic stress induced by phe- nylephrine (PE) overactivation of α1-adrenergic receptor ( α1-AR) in cardiomyocytes．
Methods:Cardiomyocytes of H9C2 rats were randomly divided into control group，PE alone treatment group，Gap26 ( Cx43 specific inhibitor) intervention group and Gap26 alone treatment group．PE alone treatment group was treated with 50 μmol / L PE for 15 min．The Gap26 intervention group was pretreated with 0. 5 μmol / L Gap26 for 30 min，and then treated with 50 μmol / L PE for 15 min．The protein and mRNA expression levels of Cx43，NLRP3 inflammasome，interleukin-1 β (IL-1 β) ，Caspase-1，interleukin-18 (IL-18 ) were detected by Western blot and qRT-PCR. The expression and co-location of Cx43 in cardiomyocytes were observed by immunofluorescence assay，and the expression of inflamma- tory cytokines IL-1 β and IL-18 in cardiomyocytes was detected by ELISA.
Results:Compared with control group， the protein and mRNA levels of Cx43，NLRP3，Caspase-1 and IL-18 in PE group increased．Compared with PE a- lone treatment group，the protein and mRNA levels of Cx43，NLRP3，Caspase-1 and IL-18 decreased after Gap26 intervention，but they were still higher than those of control group． Similarly ，immunofluorescence showed that Cx43 protein expression increased in PE alone group，while Cx43 expression was down-regulated in Gap26 inter- vention group compared with PE alone group．ELISA results showed that the expression of IL-1 β and IL-18 was sig- nificantly up-regulated in PE alone group，but down-regulated in Gap26 intervention group．
Conclusion:Cx43 is involved in α1-AR activation induced cardiac acute sympathetic stress by regulating NLRP3 inflammasome.
- Full text:2025021808445472753Cx43调控NLRP3炎性...激活诱导的心脏急性交感应激_王文博.pdf
