Functional study of PDK1 in the cerebellum of aging miceFunctional study of PDK1 in the cerebellum of aging mice
10.19405/j.cnki.issn1000-1492.2024.12.001
- Author:
Wenjie Peng
1
;
Cui Qi
1
;
Rui Liu
1
;
Jun Gao
2
Author Information
1. Dept of Neurobiology , School of Basic Medicine , Nanjing Medical University , Nanjing 211166
2. Dept of Neurobiology , School of Basic Medicine , Nanjing Medical University , Nanjing 211166； Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases , Shanghai Stomatological Hospital & School of Stomatology , Fudan University , Shanghai 200001
- Publication Type:Journal Article
- Keywords:
aging;
purkinje cells;
PDK1;
rpS6;
PKCγ;
AKT
- From:
Acta Universitatis Medicinalis Anhui
2024;59(12):2065-2071
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of 3-phosphoinositide-dependent protein kinase-1(PDK1) on the purkinje cells(PCs) in aging mice.
Methods:The footprint test, elevated beam-walk test and rotarod test were used to measure the gait traits, motor balance and motor coordination of mice. Immunoflourescence was used to detect the structure of the cerebellum and the morphology of cells in the cerebellum. The expression level of PDK1, protein kinase C gamma(PKCγ) and protein kinase B(also known as AKT) and the phosphorylation of ribosomal protein S6(rpS6) in the cerebellum were detected by Western blot.
Results:The mice showed motor defects in the overlap between forelimb and hindlimb, motor balance and motor coordination ability in an age-dependent manner. Furthermore, the cerebellum and the soma size of the PCs decreased in an age-dependent manner. However, the density of the PCs was not affected by age. Additionally, the expression level of the PDK1, PKCγ, AKT and the phosphorylation of rpS6 in the cerebellum were reduced with age.
Conclusion:The cerebellar size and the cell soma size of PCs decrease in the old mice. These effects may contribute to the reduction of the expression of PKCγ and AKT and the phosphorylation of rpS6 in the old mice, ultimately affecting the cerebellar motor coordination.
- Full text:2025021708512770526PDK1在衰老小鼠小脑中的功能研究_彭文婕.pdf
