Effects of Pseudomonas aeruginosa PmrB△Leu172 mutation on polymyxin B resistance
10.11665/j.issn.1000-5048.2023121001
- VernacularTitle:铜绿假单胞菌PmrB△Leu172缺失突变对多黏菌素B耐药的影响
- Author:
Zhen DENG
1
;
Wei HUANG
;
Xiaoqin WANG
Author Information
1. 广东医科大学机构药学院, 东莞523000
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
pmrB;
drug resistance;
polymyxin B
- From:
Journal of China Pharmaceutical University
2024;55(6):809-815
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the effects of PmrB△Leu172 on polymyxin B resistance in Pseudomonas aeruginosa. Pseudomonas aeruginosa PmrB△Leu172 strain was constructed by homologous recombination technology, and the minimum inhibitory concentration of polymyxin B against wild type and mutant strains were determined by the broth microdilution technique. The effect of PmrB△Leu172 mutation on gene transcription level was determined by RNA sequencing. Reverse transcription quantitative PCR was used to verify the effect of PmrB△Leu172 mutation on the transcription level of PmrB-regulated genes in P. aeruginosa. The results showed that the minimum inhibitory concentration and minimum bactericidal concentration of mutant strain were 4 and 2 times higher than that of wild-type, respectively, and the transcription level of PmrB-regulated genes was also up-regulated. The results suggested that PmrB△Leu172 mutation of P. aeruginosa can enhance the regulation of PmrA-PmrB two-componentsystem, which leads to the resistance of P. aeruginosa to polymyxin B.
