Study on the correlation between B7-H3 and acquired drug resistance of EGFR-TKI targeted therapy in lung adenocarcinoma
10.19405/j.cnki.issn1000-1492.2022.09.028
- Author:
Ying Yang
1
;
Meng Ding
2
;
Wei Tang
1
;
Huanhuan Liu
1
;
Xingyu Fan
1
;
Liwen Chen
1
Author Information
1. Dept of Clinical Laboratory, The Second Afiliated Hospital of Anhui Medical University,Hefei 230601
2. Dept of Clinical Laboratory, Nanjing Hospital afiliated to Nanjing University of Chinese Medicine ,Nanjing 210023
- Publication Type:Journal Article
- Keywords:
non⁃small cell lung cancer;
adenocarcinoma;
EGFR⁃TKI;
B7⁃H3;
drug resistance
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1490-1493,1498
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the correlation between co-signaling molecule B7-H3 and epidermal growth factor receptor tyrosine kinase inhibitor(EGFR-TKI) target therapy in acquired drug resistance of lung adenocarcinoma.
Methods :Immunohistochemical method was used to detect B7-H3 expression in 56 lung adenocarcinoma patients, and the cohort was divided into low and high B7-H3 expression groups.The proportion and occurrence time of acquired drug resistance after EGFR-TKI targeted therapy were compared between the two groups.Gefitinib-resistant EGFR mutant lung adenocarcinoma cell lines H3255 and HCC827 were induced and cultured in vitro.The wild-type cell lines were used as control, and the expression level of B7-H3 on resistant cell lines B7-H3 was analyzed by flow cytometry.
Results:In 56 lung adenocarcinoma patients, 31 cases and 25 cases had low and high B7-H3 expression, respectively.By the end of this study, the cases of acquired drug resistance in patients with low and high B7-H3 expression were 12 cases(38.7%) and 14 cases(56.0%)(P=0.19,χ2=1.663),and the median drug resistance time was 13.2 months and 5.1 months(P=0.004,t=3.193),respectively.Compared with wild-type H3255 and HCC827 cell lines, the expression level of drug-resistant strain B7-H3 further increased.
Conclusion:B7-H3 expression level is correlated with acquired drug resistance of EGFR-TKI targeted therapy in lung adenocarcinoma.
- Full text:2024122715454002715B7-H3与肺腺癌EGFR...治疗继发性耐药的相关性研究_杨英.pdf
