Remote ischemic preconditioning with plasma exosomes increases vascular endothelial hypoxia tolerance
10.19405/j.cnki.issn1000-1492.2024.10.016
- VernacularTitle:远隔缺血预适应血浆外泌体增加血管内皮细胞低氧耐受
- Author:
Xi ZHANG
1
;
Wenjie YANG
;
Guo SHAO
Author Information
1. 内蒙古科技大学包头医学院基础医学与法医学院,包头 014060;内蒙古科技大学包头医学院医学技术与麻醉学院,包头 014060
- Keywords:
remote ischemic preconditioning;
exosome;
endothelial cell;
hypoxia tolerance;
tight junction
- From:
Acta Universitatis Medicinalis Anhui
2024;59(10):1809-1814
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of plasma exosomes on hypoxia tolerance of human microvascular endo-thelial cells(HMEC-1)in stroke patients and healthy controls before and after remote ischemic preconditioning(RIPC)treatment.Methods HMEC-1 cells were divided into a blank control group(Group C)and oxygen glu-cose deprivation(OGD)treatment group,using plasma exosomes from stroke patients before and after RIPC(S-b group,S-a group)and healthy adults before and after RIPC(C-b group,C-a group).HMEC-1 cells were incuba-ted with a concentration of exosome(35 μg/ml)for 24 hours,followed by OGD treatment for 6 hours and reperfu-sion treatment for 24 hours in all groups.Water-soluble tetrazolium salt-1(WST-1)method was used to detect en-dothelial cell viability,caspase-3 enzyme activity was measured,and Western blot was used to detect the expression levels of zone occluden-1(ZO-1)and claudin-5.Results After RIPC in healthy adults,plasma exosomes signifi-cantly increased the cell activity of HMEC-1 cells and decreased the activity of caspase-3 enzyme in HMEC-1 cells.Conclusion After RIPC treatment,plasma exosomes reduced endothelial cell apoptosis and increased endothelial cell hypoxia tolerance.
- Full text:2024122513182878588远隔缺血预适应血浆外泌体增加血管内皮细胞低氧耐受_张晰.pdf
