C-Myc-regulated lnc-TBL1XR1-5 influences the progression of oral squamous cell carcinoma
10.19405/j.cnki.issn1000-1492.2024.09.011
- VernacularTitle:c-Myc通过lnc-TBL1XR1-5影响口腔鳞状细胞癌的发生发展
- Author:
Miaoting FU
1
;
Youming ZHU
Author Information
1. 安徽医科大学口腔医学院,合肥 230032;口腔疾病研究安徽省重点实验室,合肥 230032
- Keywords:
long non-coding RNA;
c-Myc;
oral squamous cell carcinoma;
lnc-TBL1XR1-5;
cell proliferation;
cell migration
- From:
Acta Universitatis Medicinalis Anhui
2024;59(9):1564-1572,1582
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study examined the influence of c-Myc regulated long noncoding RNA TBL1XR1-5(lnc-TBL1XR1-5)on the progression of oral squamous cell carcinoma(OSCC).Methods Based on the expres-sion of c-Myc,bioinformatics analysis was performed on head and neck squamous cell carcinoma samples in the TCGA database,and lnc-TBL1XR1-5 with closely related c-Myc expression was screened.The effects of c-Myc overexpression and knockdown on lnc-TBL1XR1-5 were detected.Expression relationship of c-Myc and lnc-TBL1XR1-5 in OSCC and para-cancer tissues.Dual-luciferase reporter assays were used to verify the binding of c-Myc to the lnc-TBL1XR1-5 promoter region.RNA FISH were used to determine the localization of lnc-TBL1XR1-5 in OSCC cell lines.The effects of overexpression,knockdown of lnc-TBL1XR1-5 on migration,metabolism,and proliferation of OSCC cells were observed by quantitative real-time polymerase chain reaction(qRT-PCR),scratch tests,transwell assays,medium color and pH changes,cell counts,CCK-8 assay,and colony formation assays.Results It was found that c-Myc positively regulated the expression of lnc-TBL1XR1-5 in OSCC cell lines and tis-sues.The binding of c-Myc to the lnc-TBL1XR1-5 promoter region was verified by dual-luciferase reporter assays.RNA FISH showed that lnc-TBL1XR1-5 was localized in the nuclei in OSCC cells.Overexpression of lnc-TBL1XR1-5 promoted migration,metabolism and proliferation in OSCC cell lines,while knockdown of it had the opposite effect.Conclusion c-Myc positively regulates lnc-TBL1XR1-5 in OSCC.Lnc-TBL1XR1-5 affect the mi-gration,proliferation,and metabolism of OSCC by influencing the tumor microenvironment.
- Full text:2024122420113903214c-Myc通过lnc-TB...响口腔鳞状细胞癌的发生发展_符妙婷.pdf
