Cytotoxicity of novel Staphylococcus aureus filtrate on non-small cell lung cancer cell
- VernacularTitle:新型金黄色葡萄球菌滤液对NSCLC细胞的毒性研究
- Author:
Xinyu LI
1
;
Qiang ZHOU
;
Liwen CHEN
Author Information
- Keywords: Staphylococcus aureus; hemolytic phenotype; filtrate; non-small cell lung cancer; cytotoxicity; anti-tumor activity
- From: Acta Universitatis Medicinalis Anhui 2024;59(9):1529-1535
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the toxicity and mechanism of a novel Staphylococcus aureus with incomplete hemolytic phenotype(SIHP)filtrate on non-small cell lung cancer(NSCLC)HCC827 cell line.Methods The filtrates and diluents of novel SIHP and Staphylococcus aureus with complete hemolytic phenotype(SCHP)were co-incubated with 1％ human red blood cells and HCC827 cells.Hemoglobin release method was used to detect red blood cell toxicity,CCK-8 method was used to detect HCC827 cell activity.Ion selective electrode method,colori-metric method and immunofluorescence method were used to detect potassium(K+),lactate dehydrogenase(LDH)and interleukin-6(IL-6)concentrations in the incubation supernatants.Results The filtrate and diluent of novel SIHP could significantly damage the activity of HCC827 cells,causing K+and LDH leakage.The filtrate and 1:3 diluent of novel SIHP resulted in a decrease and an increase in IL-6 secretion in HCC827 cells,respec-tively.Compared with SCHP,the filtrate and diluent of novel SIHP had stronger red blood cell toxicity and more severe K+leakage in HCC827 cells.Conclusion The filtrate of novel SIHP can damage the cell membrane,lead to content release and kill NSCLC cells,with a stronger toxicity than SCHP.
- Full text:2024122419095019078新型金黄色葡萄球菌滤液对NSCLC细胞的毒性研究_李欣雨.pdf
