Mechanism of GP73 neutralizing antibody inhibiting MCD diet-induced nonalcoholic fatty liver disease
10.19405/j.cnki.issn1000-1492.2022.09.011
- Author:
Jialong Liu
1
;
Xiaoli Yang
1
;
Xuemiao Zhang
2
;
Xiaopan Yang
2
;
Congwen Wei
2
Author Information
1. Clinical Laboratory of The Third Medical Center of Chinese PLA General Hospital ,Anhui Medical University, Beijing 100039; Beijing Institute of Biotechnology,Academy ofMilitary Medical Sciences ,Beijing 100071
2. Beijing Institute of Biotechnology,Academy ofMilitary Medical Sciences ,Beijing 100071
- Publication Type:Journal Article
- Keywords:
golgi protein 73;
GP73 neutralizing antibody;
non⁃alcoholic fatty liver disease;
methionine⁃choline deficient
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1398-1403
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function and mechanism of golgi protein 73(GP73) neutralizing antibody in Methionine-choline deficient(MCD) diet-induced non-alcoholic fatty liver disease(NAFLD).
Methods : Total cholesterol(TC),aspartate aminotransferase(AST),triglyceride(TG) and serum alanine aminotransferase(ALT) levels were detected after MCD diet and GP73 neutralizing antibody intervention. Lipid deposition in liver tissue was observed by oil red O staining; qRT-PCR was used to detect the gene expression ofACC1,HMGR,TIMP1andTGF-βin liver tissue; Western blotting was used to detect α-SMA and SREBP1 protein expression levels in liver tissues.
Results :GP73 neutralizing antibody could reduce the accumulation of serum ALT and AST(P<0.001) induced by MCD diet, and increase the levels of serum TG and TC(P<0.001);The gene expression levels ofHGMR(P<0.001),TGF-β(P<0.05),ACC1(P<0.01) and the protein expression levels of α-SMA(P<0.05) and SREBP1(P<0.05) in liver tissue significantly decreased, and the lipid deposition was also improved.
Conclusion : GP73 neutralizing antibody inhibits the formation of MCD-induced NAFLD by slowing down the progression of liver fibrosis and reducing the deposition of liver lipids.
- Full text:202412241555197963GP73中和抗体抑制MCD...导的非酒精性脂肪肝机制研究_刘家茏.pdf
