Whole-brain transcriptome analysis of wool sterol synthase heterozygous knockout mice
10.19405/j.cnki.issn1000-1492.2022.09.009
- Author:
Yanling Li
1
;
Yuhao Li
1
;
Xiaomei Sun
1
;
Jun Zhang
1
;
Sumei Zhang
1
;
Shengquan Zhang
1
Author Information
1. Dept of Biochemistry,School of Basic Medical Sciences ,Anhui Medical University,Hefei 230032 , China
- Publication Type:Journal Article
- Keywords:
lanosterol synthase;
gene knockout mice;
RNA⁃Seq;
enrichment analysis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(9):1389-1393
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the molecular changes of lanosterol synthase(LSS) gene dysfunction and cholesterol levels that may affect brain function.
Methods: The differentially expressed genes in LSS knockout(+/-) mice and WT C57 BL/6 mice were analyzed by RNA-SEQ,and the signal pathways involved in the differentially expressed genes were analyzed by KEGG enrichment. The differential genes were detected by real-time quantitative PCR and the protein expression of differential genes was detected by Western blot.
Results: By RNA-SEQ analysis, LSS(+/-) heterozygous knockout resulted in the difference of 320 genes in the mouse brain, including 145 up-regulated genes and 175 down-regulated genes.KEGG enrichment analysis showed that these differentially expressed genes involved in neural-activated receptor and ligand pathways, hippocampal signaling pathways and glutamate synaptic pathways.Real-time quantitative PCR showed that CCKBR and Htr5 b were up-regulated, respectively. Western blot results showed that CCKBR gene was highly expressed in the total protein of all brain tissues of LSS knockout(+/-) mice.
Conclusion:LSS heterozygotic knockout may affect brain function and animal behavior.
- Full text:2024122415413794389羊毛固醇合成酶杂合敲除小鼠全脑转录组学分析_李艳玲.pdf
