Study on specific imaging of choroidal melanoma cells by novel functionalized upconversion nanomaterials
10.3980/j.issn.1672-5123.2025.1.05
- VernacularTitle:新型功能化上转换纳米材料对脉络膜黑色素瘤细胞特异性成像的研究
- Author:
Bingxin ZHAO
1
;
Aicun FU
1
;
Xiuhong LI
1
;
Li WEI
1
;
Weiqun WANG
1
Author Information
1. Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
- Publication Type:Journal Article
- Keywords:
up-conversion nanoparticles;
plasma;
choroidal melanoma;
transferrin;
specific imaging
- From:
International Eye Science
2025;25(1):24-30
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare a novel functionalized upconversion nanomaterial UCNPs-PEG-Tf through an improved method and observe its specific imaging phenomenon to choroidal melanoma cells(OCM-1).METHODS: NaYF4:Yb/Er(Up-conversion nanoparticles, UCNPs)was Prepared and plasma was treated to carboxylate the surface; then amino polyethylene glycol and transferrin(Tf)were respectively loaded to prepare UCNPs-PEG-Tf. Characterized them accordingly, its biocompatibility was tested accordingly, and the specific fluorescence phenomenon of OCM-1 was detected by fluorescence spectrophotometer and inverted fluorescence microscopy.RESULTS: Characterization confirmed successful synthesis of UCNPs, UCNPs-PEG, and further loading of Tf to form UCNPs-PEG-Tf. UCNPs-PEG-Tf showed excellent biocompatibility and emitted significant green fluorescence. Under the same conditions, fluorescence intensity measurement and observations from the inverted fluorescence microscope both demonstrated its significant specificity in imaging to OCM-1 cells.CONCLUSION: The synthesized novel functionalized UCNPs-PEG-Tf nanocomposite showed good biocompatibility and achieve specific imaging to OCM-1 cells.
