Pharmacodynamic and Mechanism of Anti-influenza Effect of Jiawei Xiangru Oral Liquid
10.13748/j.cnki.issn1007-7693.20231733
- VernacularTitle:加味香薷口服液抗流感的药效学及作用机制
- Author:
Wei LI
1
;
Qing DU
2
;
Dan CHEN
2
;
Xiaoqing WANG
3
;
Xiaolan LI
1
;
Ke LI
1
Author Information
1. Hunan University of Chinese Medicine, Changsha 410208, China；Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China
2. Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China
3. Hunan Center for Safety Evaluation and Research of Drugs & Hunan Key Laboratory of Pharmcodynamics and Safety Evaluation of New Drugs, Changsha 410331, China
- Publication Type:Journal Article
- Keywords:
Jiawei Xiangru oral liquid ； influenza ；network pharmacology； pharmacodynamics
- From:
Chinese Journal of Modern Applied Pharmacy
2024;41(7):927-938
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE :To observe the anti-influenza effect of Jiawei Xiangru oral liquid through MDCK cells and mice, and predict its therapeutic mechanism for influenza based on network pharmacology.
METHODS
Observed the effect of different concentrations of Jiawei Xiangru oral liquid on MDCK cell damage caused by influenza A virus type 3 and its protective effect on influenza virus infected mice. The effective ingredients and action targets of each drug were collected through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Collect influenza disease related targets using GeneCards 5.14, OMMI and other databases, and construct a network diagram. Build PPI network diagrams, GO enrichment, and KEGG pathway analysis using STRING and Metascape databases. Complete molecular docking and 3D visualization display using AutoDockTools and PyMOL software.
RESULTS
In vitro experiments shown that the Jiawei Xiangru oral liquid had a significant inhibitory effect on MDCK cell damage and hemagglutination caused by influenza virus. In vivo experiments shown that it could prolong the survival time of mice infected with influenza A virus, improve the death protection rate of mice infected with the virus, and reduce the lung index and lung tissue virus titer of mice. The 82 active ingredients and 168 common targets were screened, with carotenoid, folic acid, 3'- methoxy daidzein, coellitin, and kaempferol as key components, and TNF, AKT1, EGFR, STAT3, SRC, MMP9, and PTGS2 as core targets. KEGG pathway enrichment analysis showed that the main mechanism of action maight exert anti-influenza virus effects through the TNF signaling pathway, chemokine signaling pathway, PI3K/Akt signaling pathway, and VEGF signaling pathway.
CONCLUSION
Pharmacodynamics have shown that the Jiawei Xiangru oral liquid can effectively treat influenza diseases, and its mechanism maybe related to the synergistic regulation of "multi components, multi targets, and multi pathways".
