Topical application of herbal formula for the treatment of ligature-induced periodontitis.
10.5051/jpis.2015.45.4.145
- Author:
Mi Hye KIM
1
;
You Yeon CHOI
;
Hye Ji LEE
;
Haesu LEE
;
Jung Chul PARK
;
Woong Mo YANG
Author Information
1. Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine, Seoul, Korea. wmyang@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Matrix metalloproteinase 9;
Medicinal herbs;
Osteoclasts;
Periodontitis
- MeSH:
Alveolar Bone Loss;
Angelica;
Angelica sinensis;
Animals;
Bone Resorption;
Cimicifuga;
Collagen;
Dental Cementum;
Ligation;
Mandible;
Matrix Metalloproteinase 9;
Methylene Blue;
Molar;
Osteoclasts;
Periodontitis*;
Plants, Medicinal;
Rats;
Rats, Sprague-Dawley;
Rehmannia;
RNA, Messenger;
Steam;
Zanthoxylum
- From:Journal of Periodontal & Implant Science
2015;45(4):145-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats. METHODS: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR. RESULTS: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation. CONCLUSIONS: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis. GRAPHICAL ABSTRACT:
