Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS
10.13748/j.cnki.issn1007-7693.20223494
- VernacularTitle:基于UHPLC-Q-TOF-MS/MS分析猪牙皂中化学成分
- Author:
Xiucuan JING
1
;
Junmao LI
1
;
Yulin FENG
1
;
Fei GE
1
;
Min YAO
2
;
Jiqing BAI
3
Author Information
1. Jiangxi University of Chinese Medicine, Nanchang 330004, China;
2. Jiangxi Institute for Drug Control, Nanchang 330004, China;
3. Jiangxi University of Chinese Medicine, Nanchang 330004, China;Shaanxi University of Chinese Medicine, Xianyang 712046, China;Shaanxi Chinese Medicine Raw Material Quality Testing Technology Service Center, Xianyang 712046, China
- Publication Type:Journal Article
- Keywords:
Gleditsiae Fructus Abnormalis;
zleditsia sinensis;
chemical composition;
UHPLC-Q-TOF-MS/MS
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(23):3225-3235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To Establish a liquid chromatography-mass spectrometry analysis method to comprehensively and quickly identify the chemical components in Gleditsiae Fructus Abnormalis. METHODS The ultra-high liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS) was applied for the composition analysis of Gleditsiae Fructus Abnormalis extract; its mobile phase was 0.1% formic acid(A)-acetonitrile(B) with gradient elution at a flow rate of 0.3 mL·min-1. Accurate ion mass and secondary fragmentation information were obtained in negative ion mode, combined with databases and standard reference for structure elucidation. RESULTS A total of 78 compounds(32 flavonoids, 15 phenolic acids, 22 lignans and 9 chemical constituents of monoterpenoid acids) with 14 potential new compounds were screened. CONCLUSION This study comprehensively analyzes the chemical composition of Gleditsiae Fructus Abnormalis, and provides a basis for the investigation of the pharmacodynamic basis and quality standards of this drug.
