Effect of urolithin B on the biological behavior of glioblastoma U251 cell
10.19405/j.cnki.issn1000-1492.2022.02.005
- Author:
Cuilan Liu
1
;
Di Zhao
1
;
Juanjuan Dai
2
;
Dan Wang
1
;
Chen Li
1
;
Song Liu
3
Author Information
1. The Institute for Metabolic and Neuropsychiatric Disorders , Binzhou Medical University Hospital , Binzhou 256603
2. Cancer Research Institute , Binzhou Medical University Hospital , Binzhou 256603
3. Dept of Thyroid and Breast Surgery, Binzhou Medical University Hospital , Binzhou 256603
- Publication Type:Journal Article
- Keywords:
Urolithin B;
U251cell;
proliferation;
migration;
invasion;
apoptosis;
cell cycle
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):187-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect and mechanism of Urolithin B ( UB) on proliferation , migration , invasion , apoptosis and cell cycle of glioblastoma cells.
Methods :The effects of UB on the proliferation , migration and invasion of glioblastoma U251 cells were detected by CCK⁃8 , clone formation assay , scratch healing assay , Transwell invasion assay respectively. The regulation effect of UB on cell cycle and apoptosis was detected by flow cytometry. Western blot was used to detect the effects of UB on the phosphorylation levels of downstream signaling pathway proteins ERK , AKT , p38 and JNK.
Results :Compared with the control group , at 24 h and 48 h , the absorbance value of U251 cells was decreased by UB (P < 0. 01) in a dose dependent manner. UB reduced the percentage of cell clone formation (P < 0. 01) . The percentage of the scratch healing area was reduced by UB (P < 0. 05 or P < 0. 01) . The percentage of the number of invaded cells was reduced by UB (P < 0. 01) . UB could induce apoptosis (P < 0. 01) and cause the cells to stagnate in G2/M phase (P < 0. 01) . UB could significantly inhibit the phosphorylation of ERK (P < 0. 05 or P < 0. 01) and AKT (P < 0. 05 or P < 0. 01) .
Conclusion :UB can inhibit proliferation , migration , invasion , apoptosis and cell cycle of glioblastoma cells , and regulate the phosphorylation levels of ERK and AKT.
- Full text:2024120510422731723尿石素B对胶质母细胞瘤U251细胞生物学行为的影响_刘翠兰.pdf
