6 ⁃gingerol relieves cell apoptosis , oxidative stress and neuroinflammation in rats with Alzheimer disease by activating Wnt/β⁃catenin signaling pathway
10.19405/j.cnki.issn1000-1492.2022.01.018
- Author:
Jiao Li
1
;
Youyuan Xiao
1
;
Qin Xie
1
;
Tianrong Ma
1
;
Jianping Li
1
;
Jun Duan
1
;
Mao Su
2
Author Information
1. Dept of Geriatrics , Sichuan Academy of Medical Sciences , Sichuan People ′s Hospital , Chengdu 610072
2. Dept of Rehabilitation Medicine ,Sichuan Academy of Medical Sciences , Sichuan People ′s Hospital , Chengdu 610072
- Publication Type:Journal Article
- Keywords:
6 ⁃gingerol;
AD model rat;
apoptosis;
oxidative stress;
neuroinflammation;
Wnt/β⁃catenin signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):95-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of 6⁃gingerol on amyloid β ⁃protein(Aβ) induced apoptosis , oxidative stress and neuroinflammation in Alzheimer's disease(AD) rats by regulating Wnt/β⁃catenin signaling pathway.
Methods : The rat model of AD was established by injecting Aβ into hippocampus of bilateral brain. The rats were
randomly divided into six groups : control group , model group , low⁃dose 6⁃gingerol group , medium⁃dose 6⁃gingerol group , high⁃dose 6 ⁃gingerol group and positive control group. Nerve injury was evaluated by nerve injury score , brain water content was measured by dry/wet method , and hippocampal histological injury was measured by HE staining. The apoptosis of hippocampal cells was detected by flow cytometry and TUNEL staining. The protein levels of iNOS , IL⁃6 , TNF⁃α , SOD , GSH⁃Px and MDA were detected by ELISA. The mRNA and protein relative expression levels of Wnt and β ⁃catenin were detected by qRT⁃PCR and Western blot.
Results :Compared with the model group , after 6⁃gingerol treatment , nerve injury score decreased , brain water content decreased , the apoptosis rate decreased , the expression of iNOS , IL⁃6 and TNF⁃α decreased , SOD and GSH⁃Px protein content increased , while MDA protein content decreased. The mRNA and protein relative expression levels of Wnt and β ⁃catenin were up⁃regulated.
Conclusion:6 ⁃gingerol can reduce apoptosis , oxidative stress and neuroinflammation in AD rats by activating Wnt/β⁃catenin signaling pathway.
- Full text:20241203093147235996-姜酚通过调节Wnt_β...、氧化应激和神经炎症的影响_李交.pdf
