Analysis of the biosynthesis pathways of phenols in the leaves of Tetrastigma hemsleyanum regulated by supplemental blue light based on transcriptome sequencing
10.16438/j.0513-4870.2024-0070
- VernacularTitle:基于转录组测序的蓝光补光调控三叶青叶片酚类成分生物合成途径分析
- Author:
Hui-long XU
1
;
Nan YANG
1
;
Yu-yan HONG
1
;
Meng-ting PAN
1
;
Yu-chun GUO
2
;
Shi-ming FAN
1
;
Wen XU
1
Author Information
1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2. College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
- Publication Type:Research Article
- Keywords:
italic>Tetrastigma hemsleyanum;
transcriptome;
supplemental blue light;
ifferentially expressed gene;
biosynthesis of phenols
- From:
Acta Pharmaceutica Sinica
2024;59(10):2864-2870
- CountryChina
- Language:Chinese
-
Abstract:
Analyze the changes in phenolic components and gene expression profiles of Tetrastigma hemsleyanum leaves with supplemental blue light, and screen differentially expressed genes related to phenolic metabolism, providing a basis for improving the quality of T. hemsleyanum leaves. Using the leaves of T. hemsleyanum under supplemental blue light and visible light as research materials, the content of phenolic components such as neochlorogenic acid, chlorogenic acid, and 3-O-coumaroyl quinic acid was significantly increased by supplemental blue light. A total of 102 949 unigenes were obtained from the transcriptome, including 14 564 differentially expressed unigenes. They can be divided into 52 subclasses by gene ontology (GO) function, kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed that these differentially expressed unigenes were significantly enriched in phenolic metabolic pathways such as phenylpropane biosynthesis, flavonoid biosynthesis and isoflavone biosynthesis. A total of 63 differentially expressed unigenes were identified in the pathways related to the biosynthesis of phenolic cpmponents, including 19 key enzymes such as phenylalanine ammonia-lyase (PAL), cinnamyl-alcohol dehydrogenase (CAD), flavonol synthase (FLS), and so on. This study greatly enriched the genetic data information of T. hemsleyanum and laid a foundation for analyzing the mechanism of blue light promoting the biosynthesis of phenols and molecular breeding of T. hemsleyanum.
