Preparation of Salmonella typhimurium VNP20009 conjugated with imidazole-zeolite framework and its anti-tumor effect
10.16438/j.0513-4870.2024-0045
- VernacularTitle:搭载咪唑-沸石骨架的减毒沙门氏菌的制备及抗肿瘤作用研究
- Author:
Xiao-fang ZHONG
1
;
Xiao-yu DENG
1
;
Shuai LIU
2
Author Information
1. School of Pharmacy, Guangdong Medical University, Dongguan 523109, China
2. Huangjiang Hospotial, Dongguan 523750, China
- Publication Type:Research Article
- Keywords:
imidazole-zeolite framework;
italic>Salmonella VNP20009;
biomimetic mineralization;
proliferation;
B16F10 cell
- From:
Acta Pharmaceutica Sinica
2024;59(6):1841-1846
- CountryChina
- Language:Chinese
-
Abstract:
In this study, inspired by biomimetic mineralization process, we have developed imidazole-zeolite framework (ZIF-8) conjugated VNP20009, and chemotherapy drug doxorubicin hydrochloride (DOX) was encapsulated to obtain ZD@VNP. The morphology and the combination of ZIF-8 and VNP was characterized by transmission electron microscopy and laser confocal microscopy. Fluorescence spectrophotometry was used to examine the encapsulation rate and in vitro release rate of DOX. CCK-8 and FDA/PI cell viability staining experiments were used to evaluate the ability of ZD@VNP to inhibit cell proliferation. Melanoma mouse model was established to investigate the effect of ZD@VNP to inhibit tumor growth. It was shown that ZIF-8 was evenly bounded to the surface of VNP20009, and laser confocal microscopy results also confirm the combination of ZIF-8 with VNP in ZD@VNP. The encapsulation rate of DOX in ZD@VNP was calculated to be 85.7% ± 3.7%, and the release of DOX under the buffer at pH 6.0 was significantly higher than that of pH 7.4. ZD@VNP treatment resulted in a greater inhibitory effect on B16F10 cell proliferation compared to DOX treatment. Animal experiment results showed that compared with VNP+DOX, ZD@VNP treatment can significantly inhibit the growth of B16F10 tumors in C57BL/6 mice and prolonged survival (all animal experiments were approved by the Institutional Animal Care and Ethics Committee Guangdong Medical University, No. GDMU-2023-2518). In summary, ZD@VNP was prepared through a facile, one-step method, which can significantly enhance the proliferation inhibitory effect of DOX to inhibit tumor growth and prolong survival. Our results demonstrate that ZD@VNP has great application prospects in the field of drug delivery.
