Changes of arachidonic acid metabolic pathway and intervention effect of salvianolic acid salt in OLV induced lung inj ury in rabbits
10.19405/j.cnki.issn1000-1492.2022.01.005
- Author:
Hua Chen
1
;
Bosheng Zhang
1
;
Xiaoping Long
2
Author Information
1. Dept of Traditional Chinese Medicine , The First Afiliated Hospital of Nanhua University, Hengyang 421001
2. Pulmonary and Critical Care Medicine , The First Afiliated Hospital of Nanhua University, Hengyang 421001
- Publication Type:Journal Article
- Keywords:
salvianolic acid salt;
one lung ventilation;
cyclooxygenase⁃2;
5 ⁃lipoxygenase;
Clara cell secretory pro⁃ tein;
Cytoplasmic phospholipase A2
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the protective effect and mechanism of salvianolic acid salt on acute lung injury
induced by one lung ventilation(OLV) in rabbits.
Methods : 24 rabbits were randomly divided into three groups : control group , model group and treatment group , with 8 rabbits in each group. The W/D value of lung was measured , and the morphological changes of lung tissue were observed by HE staining and electron microscopy; The contents of arachidonic acid(AA) , leukotriene B4( LTB4) , thromboxaneA2( TXA2) and prostacyclin 2( PGI2) in rabbit lung tissue were detected by ELISA ; The expressions of cyclooxygenase⁃2(COX⁃2) ,5 ⁃lipoxygenase(5 ⁃LOX) in lung tissue were detected by RT⁃qPCR; The expressions of COX⁃2 , 5 ⁃LOX , clara cell secretory protein(CCSP) and cytoplasmic phospholipase A2(C⁃PLA2) in lung tissue were detected by Western blot.
Results :The W/D ratio of model group was higher than that of control group , but decreased after treatment ( P < 0. 05) ; HE staining showed that in the model group , the capillaries were dilated and congested , there were red exudates in the alveoli , the walls of alveoli were thickened , and inflammatory cells were infiltrated , but the treatment of salvianolic acid salt was relieved ; The mitochondria swelling , crista breaking and even disappearing were observed by transmission electron microscope in the model group , and alveolar epithelial cell Ⅰ (AT⁃ Ⅰ ) ultrastructural damage in the treatment group significantly improved compared with that in the model group ; The AA , LTB4 , TXA2 and PGI2 contents in the treatment group were lower than those in the model group (P < 0. 05) . RT⁃qPCR showed that the expression of 2 and 5 ⁃LOX decreased after Salvianolic acid salt treatment (P < 0. 05) ; Western blot showed that the expression of COX⁃2 , 5 ⁃LOX and C ⁃PLA2 in treatment group was lower than that in model group , while the expression of CCSP increased (P < 0. 05 ) .
Conclusion :Salvianolic acid salt has protective effect on lung injury induced by one⁃lung ventilation. Its mechanism may be related to down⁃regulation of COX⁃2 , 5 ⁃LOX and C ⁃PLA2 expression , up⁃regulation of CCSP expression.
- Full text:2024102517041028024单肺通气诱发兔肺损伤时花生...化及丹参多酚酸盐的干预研究_陈华.pdf
