Effect of c-Myc-regulated lnc-CCDC117-1 on the progression of tongue squamous cell carcinoma
10.19405/j.cnki.issn1000-1492.2023.06.021
- Author:
Qingying Shi
1
;
Youming Zhu
1
Author Information
1. School of Stomatology，Anhui Medical University，Laboratory of Anhui Oral Disease Research Center，Hefei 230032
- Publication Type:Journal Article
- Keywords:
ong non-coding RNA;
c-Myc;
tongue squamous cell carcinoma;
lnc-CCDC117-1;
cell proliferation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):1001-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To clarify the targeted regulatory relationship between long non-coding RNA ( lncRNAs) lnc-CCDC117-1 and oncogenic transcription factor c-Myc，and to explore the effect of lnc-CCDC117-1 knockdown and overexpression on the development of tongue squamous cell carcinoma cells．
Methods : Lentiviral vectors car- rying flag-c-Myc，plko．1-shc-Myc and their controls were transfected into HN6，SCC9 and CAL27 cells respective- ly，and real-time quantitative PCR(qRT-PCR) was used to detect the expression of lnc-CCDC117-1．In addition， the intracellular localization of lnc-CCDC117-1 was detected by fluorescence in situ hybridization．On this basis，the lnc-CCDC117-1 expression vector and knockdown vector were constructed and transfected with HN6 ，SCC9， CAL27 cells，CCK-8 method，cloning and formation to verify the proliferation of tongue squamous cell.
Results:LncRNAs positively regulated by c-Myc were initially screened by gene chip technology．After overexpression or c- Myc knockdown in tongue squamous cell HN6，these lncRNAs were verified to be consistent with the results of the chip，and the expression difference of lnc-CCDC117-1 was found to be the most significant．qRT-PCR test showed that c-Myc had a positive regulatory effect on lnc-CCDC117-1．When c-Myc was overexpressed，the expression of lnc-CCDC117-1 could be significantly up-regulated．lnc-CCDC117-1 expression was significantly down-regulated by c-Myc knockdown．Dual luciferase reporter genes showed that c-Myc could target the regulation of lnc-CCDC117-1， and c-Myc was involved in regulating and enhancing the transcriptional activity of lnc-CCDC117-1．lnc-CCDC117- 1 mainly existed in the nucleus of the cell．qRT-PCR results showed that the expression of lnc-CCDC117-1 and c- Myc significantly decreased by sh-lnc-CCDC117-1．The expression of lnc-CCDC117-1 was significantly up-regula- ted by + lnc-CCDC117-1．The results of growth curve assay，CCK-8 assay，cell scratch assay and cloning forma- tion showed that overexpression of lnc-CCDC117-1 significantly promoted the proliferation and migration of tongue squamous cell cells，while knockdown of lnc-CCDC117-1 could inhibit the growth and proliferation of tongue squa- mous cell carcinoma cells．
Conclusion :lnc-CCDC117-1 is positively regulated by c-Myc，and overexpression of lnc-CCDC117-1 can promote cell proliferation，on the contrary，inhibit cells growth.
- Full text:2024101509212797136c-Myc调控lnc-CC...17-1对舌鳞癌进展的影响_石清影.pdf
