The expression of miR⁃381⁃3p in acute myeloid leukemia and its effect on the proliferation and apoptosis of leukemia cells
10.19405/j.cnki.issn1000-1492.2023.07.017
- Author:
Hongxia Zhang
1
;
Kui Wang
1
;
Guangsheng Wu
1
Author Information
1. Dept ofHematology , The First Afiliated Hospital of Shihezi University , Shihezi 832000
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia;
miR⁃381 ⁃3p;
prognosis;
proliferation;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1164-1170
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the expression , clinical significance , progression , and prognosis of miR⁃381 ⁃3p in acute myeloid leukemia (AML) , as well as its impact on AML cell proliferation and apoptosis , in order to provide theoretical basis for the treatment of AML.
Methods :Bioinformatics analysis was used to identify differentially expressed miRNAs , clinical data and blood samples of AML patients were collected , and the expression levels of miRNAs in the bone marrow fluid of the included patients were measured to further elucidate the relationship between miRNAs and AML. The included patients were followed up to calculate overall survival (OS) and disease⁃free survival (DFS) ; AML cells were cultured in vitro , miR⁃381 ⁃3p plasmids were constructed , miR⁃381 ⁃3p was overexpressed and miR⁃381 ⁃3p was knocked down in AML , and they were divided into five groups : control , miR⁃381 mimics , mimics NC , miR⁃381 inhibitor , inhibitor NC . The proliferation and apoptosis of AML cells were detected using CCK⁃8 and flow cytometry .
Results :Differentially expressed miRNAs were identified using bioinforDifferentially expressed miRNAs were identified using bioinfor included . The expression level of miR⁃381 in AML patients was lower than that in the control group , and all FAB subtypes were lower than those in the normal group . The expression level of miR⁃381 was not related to the age , gender , peripheral blood leukocytes , lymphocytes , and FAB typing of AML patients , and the OS and DFS of miR⁃381 patients with high expression were significantly prolonged , with statistically significant differences . In vitro experiments had shown that knocking down miR⁃381 could inhibit apoptosis and promote proliferation of AML cells . Overexpression of miR⁃381 could promote apoptosis and inhibit proliferation of AML cells .
Conclusion :MiR⁃381 ⁃ 3p is low expressed in AML patients , and its overexpression can significantly prolong OS and DFS . miR⁃381 ⁃3p can promote apoptosis of AML cells , inhibit proliferation , and may become a targeted molecule for the treatment of AML.
- Full text:2024101016430014553miR-381-3p在急性...白血病细胞增殖和凋亡的影响_张红霞.pdf
