AKT3 reverses the synergistic inhibition of miR⁃22⁃3p/29a⁃3p on LX⁃2 cell activation
10.19405/j.cnki.issn1000-1492.2023.07.012
- Author:
Ronghua Zhang
1
;
Yanan Zhang
1
;
Xiangyang Han
1
;
Xiaoyan Cui
1
;
Xiaoli Tian
2
;
Guangling Zhang
3
;
Zhiyong Liu
4
Author Information
1. School of Basic Medical Sciences ,North China University of Science and Technology, Hebei Key Laboratory for Chronic Diseases , Tangshan 063210
2. Paraplegia Sanatorium of Tangshan , Tangshan 063000
3. School of Clinical Medicine ,North China University of Science and Technology,Hebei Key Laboratory of Medical⁃Industrial Integration Precision Medicine , Tangshan 063000
4. Health Science Center of North China University of Science and Technology, Tangshan 063210
- Publication Type:Journal Article
- Keywords:
LX⁃2;
serine/threonine kinase 3;
miR⁃22⁃3p;
miR⁃29a⁃3p;
proliferation;
migration
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1132-1139
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate whether serine/threonine kinase 3 ( AKT3) is able to reverse the synergistic
inhibition of miR⁃22⁃3p/29a⁃3p on the activation of human hepatic stellate cell LX⁃2.
Methods :TargetScan , Starbase , miRDB and DIANA were used to predict the common target genes of miR⁃22⁃3p and miR⁃29a⁃3p , and the base , miRDB and DIANA were used to predict the common target genes of miR⁃22⁃3p and miR⁃29a⁃3p , and the tein⁃protein interaction (PPI) . The binding free energy of candidate target genes with two miRNAs was analyzed by RNAhybrid , and their targeting relationship was determined by double luciferase reporting experiment. CCK⁃8 , Transwell and qRT⁃PCR were used to detect the proliferation , migration and the expression of fibrosis markers of LX-2 cells.
Results :There were 24 common target genes of miR⁃22⁃3p and miR⁃29a⁃3p , and they were enriched in the hepatic fibrosis⁃related signaling pathways and biological processes. The binding free energy analysis of candidate target gene AKT3 with miR⁃22⁃3p and miR⁃29a⁃3p combined with the double luciferase experiment results showed that AKT3 was the common target gene of miR⁃22⁃3p and miR⁃29a⁃3p. The proliferation , migration and the mRNA expression of fibrosis markers of α ⁃smooth muscle actin ( α ⁃SMA) and type I collagen α1 chain (COL1A1) of LX⁃2 cells were inhibited by miR⁃22⁃3p and miR⁃29a⁃3p mimics independently or cooperatively ( P < 0. 05 ) , and AKT3 overexpression could reverse the synergistic inhibition of miR⁃22⁃3p and miR⁃29a⁃3p mimics on LX⁃2 ells mentioned⁃above (P < 0. 05) .
Conclusion :The overexpression of AKT3 can reverse the synergistic inhibition of miR⁃22⁃3p and miR⁃29a⁃3p on the activation of LX⁃2 cells.
- Full text:2024101010191973183AKT3逆转miR-22-...-2细胞活化的协同抑制作用_张荣花.pdf
