Bioinformatics-based analysis of ADCY1 gene expression in clear cell renal cell carcinoma and its clinical significance
10.3969/j.issn.1009-8291.2024.06.014
- VernacularTitle:基于生物信息学分析ADCY1基因在肾透明细胞癌中的表达及临床意义
- Author:
Lin ZHANG
1
;
Dongwen WANG
Author Information
1. 山西医科大学第一临床医学院;山西医科大学第一医院泌尿外科,山西太原 030000
- Keywords:
clear cell renal cell carcinoma;
ADCY1;
bioinformatics analysis;
prognostic marker;
therapeutic target
- From:
Journal of Modern Urology
2024;29(6):547-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression level,clinical significance and prognostic value of adenylate cyclase 1(ADCY1)in clear cell renal cell carcinoma(ccRCC).Methods ADCY1 expression level and correlation with pathological features were analyzed with UALCAN database.The prognostic value of ADCY1 in ccRCC was analyzed with GEPIA2 database.The protein expression level and three-dimensional structure of ADCY1 were analyzed with HPA database.The subcellular localization of ADCY1 was analyzed with Genecard database.ADCY1 interacting proteins were obtained using the STRING database and enriched with R software.Mutations of ADCY1 gene were analyzed with cBioPortal database.Immune cell infiltration of ADCY1 in ccRCC was analyzed with R language.ADCY1 expression level in ccRCC tissues was verified with quantitative real-time polymerase chain reaction(qRT-PCR)and immunohistochemical staining.Results ADCY1 was down-regulated in ccRCC and significantly correlated with pathological staging and grading.Patients with low ADCY1 expression had poorer overall and disease-free survival.ADCY1 was mainly expressed in the plasma membrane,extracellular,and nucleus of the cells.ADCY1 was closely related to morphine addiction,regulation of circadian rhythm,dopaminergic synapses,and cAMP signaling pathway.The main mutation type of ADCY1 in ccRCC was missense mutation.ADCY1 was positively correlated with CD4 T cell and macrophage infiltration in ccRCC.The gene and protein expressions of ADCY1 were decreased in ccRCC tissues.Conclusion ADCY1 is a new prognostic marker and potential clinical therapeutic target for ccRCC.
