Identification of chemical constituents of modified Yupingfengsan formula
- VernacularTitle:玉屏风散加味方化学成分鉴定
- Author:
Cuizhen LIU
1
;
Siqi FENG
1
;
Xiujuan LIN
1
;
Qin LU
1
;
Yusheng HUANG
2
;
Lijuan ZHANG
3
;
Detang LI
4
Author Information
1. School of First Clinical Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Lingnan Medical Research Center，Guangzhou University of Chinese Medicine，Guangzhou 510407，China;Guangdong Clinical Research Academy of Chinese Medicine，Guangzhou 510405，China
3. Guangdong Clinical Research Academy of Chinese Medicine，Guangzhou 510405，China;Dept. of Otorhinolaryngology，the First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China
4. Guangdong Clinical Research Academy of Chinese Medicine，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
modified Yupingfengsan formula;
chemical constituents;
flavonoids;
phenylpropanoids;
fragmentation pattern
- From:
China Pharmacy
2024;35(18):2225-2231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify the chemical constituents of the modified Yupingfengsan formula. METHODS UPLC-Q- Exactive Orbitrap-MS technology was adopted. The separation was performed on Waters BEH C18 column with acetonitrile （A）-0.1% formic acid solution （B） as mobile phase for gradient elution. The heating electrospray ionization was used for positive and negative ion mode scanning. The scanning range was m/z 50-1 500， and the spray voltage was 2 kV （positive ion mode） and 1.5 kV （negative ion mode）. The information of chemical constituents of modified Yupingfengsan formula was collected through literature review to establish a database； the structure of the constituent was identified based on the above database， relevant literature， and chromatography and mass spectrometry information of reference standards. RESULTS & CONCLUSIONS Totally 114 chemical constituents were identified from modified Yupingfengsan formula， including 31 flavonoids， 39 phenylpropanoids， 5 saponins， 8 terpenoids， 3 chromones， 3 curcuminoids， etc. Based on the comparison of reference standards， 8 constituents were ultimately determined， including magnoflorine， calycosin， calycosin glycoside， cimifugin， 5-O-methylvisammioside， sec-O- glucosylhamaudol， luteolin and mangiferin. These constituents mainly involved glycosylation cleavage， retro Diels-Alder fragmentation， glycosylation loss， neutral molecule loss and other fragmentation pathways.
