Effect of different storage media on elemental analysis and microhardness of cervical cavity margins restored with a bioactive material
- Author:
Hoda Saleh ISMAIL
1
;
Brian Ray MORROW
;
Ashraf Ibrahim ALI
;
Rabab Elsayed Elaraby MEHESEN
;
Salah Hasab MAHMOUD
;
Franklin GARCIA-GODOY
Author Information
- Publication Type:Research Article
- From:Restorative Dentistry & Endodontics 2024;49(1):e6-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objectives:This study aimed to investigate the elemental analysis and microhardness of a bioactive material (Activa) and marginal tooth structure after storage in different media.
Materials and Methods:Fifteen teeth received cervical restorations with occlusal enamel and gingival dentin margins using the tested material bonded with a universal adhesive, 5 of them on the 4 axial surfaces and the other 10 on only the 2 proximal surfaces. The first 5 teeth were sectioned into 4 restorations each, then stored in 4 different media; deionized water, Dulbecco's phosphate buffered saline (DPBS), Tris buffer, and saliva. The storage period for deionized water was 24 hours while it was 3 months for the other media. Each part was analyzed by scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analysis for different substrates/distances and the wt% of calcium, phosphorus, silica, and fluoride were calculated. The other 10 teeth were sectioned across the restoration, stored in either Tris buffer or saliva for 24 hours or 3 months, and were evaluated for microhardness of different substrates/ areas. Data were analyzed using analysis of variance and Tukey’s post hoc test.
Results:Enamel and dentin interfaces in the DPBS group exhibited a significant increase in calcium and phosphorus wt%. Both silica and fluoride significantly increased in tooth structure up to a distance of 75 μm in the 3-month-media groups than the immediate group.Storage media did not affect the microhardness values.
Conclusions:SEM-EDS analysis suggests an ion movement between Activa and tooth structure through a universal adhesive while stored in DPBS.
