Effect of TBC1D5 on hepatocellular carcinoma progression via JAK/STAT pathway
10.19405/j.cnki.issn1000-1492.2024.08.011
- VernacularTitle:TBC1D5通过JAK/STAT通路对肝细胞癌进展的影响
- Author:
Haowei WEI
1
;
Xuewen TAO
;
Decai YU
Author Information
1. 南京大学医学院附属鼓楼医院肝胆与肝移植外科,南京 210008;安徽医科大学第一附属医院肝胆胰及移植外科,合肥 230022
- Keywords:
hepatocellular carcinoma;
TBC1D5;
proliferation;
invasion;
JAK/STAT pathway
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1361-1369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Tre2-Bub2-Cdc16 1 domain family member5(TBC1D5)in the de-velopment of hepatocellular carcinoma(HCC).Methods Western blot(WB),Immunohistochemistry(IHC)and quantitative real-time PCR(qPCR)were used to verify the difference in TBC1D5 expression in clinical sam-ples.The HCC cell lines MHCC97H and Hep3B were chosen to construct the knockdown model.The effects on cell proliferation were detected by cell proliferation assay,colony formation assay and EdU assay.Wound assay and Transwell assay were used to detect cell migration and invasion.Flow cytometry was used to detect the changes of cell cycle and H2O2-induced apoptosis of HCC cells.Finally,the effects of knockdown and overexpression of TBC1D5 on JAK/STAT pathway were detected by WB.Results The results of WB,IHC and qPCR showed that the expression of TBC1D5 in HCC tissues was higher at the protein level(P＜0.000 1)and mRNA level(P＜0.01)than that in corresponding adjacent tissues.Compared with the control group,the proliferation level of HCC cells with TBC1D5 knockdown was decreased(P＜0.05),the formation of plate colony number decreased(P＜0.001),and the proportion of EdU-positive cells decreased(P＜0.001).The results of scratch assay and Tran-swell assay showed that the migration(P＜0.01)and invasion ability(P＜0.01)of HCC cells after TBC1D5 knockdown were significantly lower than those in the control group.After TBC1D5 knockdown,the cell cycle of HCC cells was slowed down(P＜0.05)and the ability to resist apoptosis was reduced(P＜0.01)than those in the control group.Compare with the control group,knockdown of TBC1D5 decreased the phosphorylation level of JAK and STAT proteins and inhibit the JAK/STAT pathway.Conclusion TBC1D5 is highly expressed in HCC.After knocking down TBC1D5,the proliferation,migration and invasion ability,cell cycle rate and anti-apoptosis ability of HCC cells decreased and may affect HCC progression through the JAK/STAT pathway.
- Full text:2024091412015516037TBC1D5通过JAK_S...T通路对肝细胞癌进展的影响_韦豪伟.pdf
