Construction of porous titanium surface loaded with Silane and BMP⁃2 and evaluation of its osteogenic activity
10.19405/j.cnki.issn1000-1492.2023.08.030
- Author:
Wei Li
1
;
Chongying Jiang
1
;
Lei Zhang
1
;
Xiaoyu Sun
1
;
Xin He
1
;
Biao Hong
1
;
Nan Cheng
1
;
Xiangyang Li
1
Author Information
1. Stomatological College of Anhui Medical University, The Afiliated Stomatological Hospital of Anhui Medical University,Key Lab. of Oral Diseases Research of Anhui Province , Hefei 230032
- Publication Type:Journal Article
- Keywords:
implant;
nano⁃porous structure;
Silane;
bone morphogenetic protein⁃2;
osteoblast
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1423-1427
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the preparation method of silane and bone morphogenetic protein⁃2 (BMP⁃2) on the nano⁃porous titanium surface and evaluate the osteogenic ability of the prepared surface.
Methods :Silane and BMP⁃2 modified nano⁃porous titanium surfaces were constructed by alkali⁃heat treatment , silanization modification and BMP⁃2 grafting. Scanning electron microscopy , X ⁃ray photoelectron spectroscopy , infrared spectroscopy and water contact angle tests were used to characterize the changes of surface morphology , elements , functional groups and hydrophilicity during the preparation process. Immunofluorescence was used to observe the BMP⁃2 grafted on the surface. Cell activity test and cell fluorescence staining were used to analyze the ability to promote osteoblast adhesion and proliferation , and alkaline phosphatase activity was used to evaluate the ability to promote cell osteogenesis.
Results : Material characterization results confirmed that silane and BMP⁃2 were successfully immobilized on the nano⁃porous titanium surface. Cell evaluation confirmed that the prepared surface not only significantly enhanced the adhesion and proliferation of osteoblasts , but also greatly enhanced the osteogenic ability of osteoblasts.
Conclusion :The constructed surface has better compatibility with osteoblasts and is expected to improve the osseointegration ability of titanium⁃based implants.
- Full text:202408191118180958载硅烷和BMP-2的多孔钛表面构建及其成骨活性评价_李为.pdf
