Study on the effect of Bmp2 on the osteogenic ability of adipose stem cells
10.19405/j.cnki.issn1000-1492.2023.06.015
- Author:
Wentao Lai
1
;
Zhijun Zhao
2
;
Chunyang Zhang
2
Author Information
1. Baotou Medical College，Inner Mongolia University of Science and Technology，Baotou 014010 ;Dept of Neurosurgery，The First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology，Baotou 014010
2. Dept of Neurosurgery，The First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology，Baotou 014010;Engineering Technology Center for Bone Tissue Regeneration and Injury Repair in Inner Mongolia Autonomous Region，Baotou 014010
- Publication Type:Journal Article
- Keywords:
adipose-derived stem cells;
Bmp2;
osteogenic differentiation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):863-869
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of osteogenic genes and osteogenic capacity after overexpression of bone morphogenetic protein 2(Bmp2) in rat adipose stem cells．
Methods:Bmp2 was transfected into rat adipose stem cells by electrotransfection，and the blank group was used as control．The cells were divided into blank group ( Control group) ，empty load group (Vector group) ，osteogenesis induction group (Inducement group) ，and overexpression group (Bmp2 group) ．Western blot，qRT-PCR and immunofluorescence were used to compare the expression of osteogenic genes，and alkaline phosphatase staining and alizarin red staining were used to observe cobalt sulfide precipitation and calcium salt deposition.
Results :Western blot and qRT-PCR showed that the expression of Bmp2，Bmp4，OPN，OCN，Runx2，P-Smad1 /5，Dlx2，and BSP was significantly higher compared to the Control group (P ＜0. 05 ) ，while the expression of Smad1 /5 was not statistically different． Immunofluorescence showed that the fluorescence intensity of OPN and Runx2 was significantly higher compared to the Control group (P ＜0. 05) ．The alizarin red staining and alkaline phosphatase staining showed that the Bmp2 group had increased pericellular calcium salt deposition，increased cobalt sulfide precipitation，and enhanced alkaline phosphatase activity compared to the Control group．
Conclusion :Overexpression of Bmp2 enhances osteogenic gene expression in adipose stem cells and improves their osteogenic ability.
- Full text:2024081617433183967Bmp2影响脂肪干细胞成骨能力的研究_赖文涛.pdf
