AMD3100 reverses Sorafenib resistance by down-regulating CA9 and CXCR4 expression
10.19405/j.cnki.issn1000-1492.2023.06.007
- Author:
Jiwei Qin
1
;
Hao Zheng
1
;
Zhijun Xu
1
;
Xuefeng Li
1
;
Cheng Sun
1
Author Information
1. Organ Transplantation Center，The First Affiliated Hospital of University of Science and Technology of China，Hefei 230001
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
AMD3100;
carbonic anhydrase IX;
chemokine receptor 4;
Sorafenib;
drug re- sistance
- From:
Acta Universitatis Medicinalis Anhui
2023;58(6):919-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the mechanism of AMD3100 reversing the resistance of human hepatocellular carcinoma cells to Sorafenib by regulating carbonic anhydrase IX ( CA9) and chemokine receptor 4 ( CXCR 4 ) .
Methods :The Sorafenib resistant cell lines Huh7 / Sor and HepG2 / Sor were established from human hepatocellular carcinoma cells Huh7 and HepG2．The effects of Sorafenib alone or in combination with AMD3100 on the proliferation of Huh7，HepG2，Huh7 / Sor，HepG2 / Sor cells were detected．The difference of invasive ability between Sorafenib resistant cells and non-resistant cells，and the effect of AMD3100 on the invasive ability of hepatocellular carcinoma cells were observed．And the regulation effect of Sorafenib alone or in combination with AMD3100 on the expression of CA9 and CXCR4 proteins in Huh7，HepG2，Huh7 / Sor and HepG2 / Sor cells was detected.
Results:Compared with the Control group，AMD3100 (50 μmol / L) increased the inhibitory effect of Sorafenib on the proliferation of Huh7 / Sor and HepG2 / Sor cells (P＜0．05) ．Compared with Huh7 cells，the invasive ability of Sorafenib resistant Huh7 / Sor cells was significantly enhanced (P＜0. 05) ，and AMD 3100 (50 μmol / L) decreased the invasive ability of Huh7 and Huh7 / Sor cells (P＜0．05) ．Compared with Huh7 and HepG2 cells，the protein expression of CA9 and CXCR4 in Huh7 / Sor and HepG2 / Sor cells increased (P＜0. 05) ．AMD3100 (50 μmol / L) down-regulated the protein expression of CA9 and CXCR4 in Huh7，HepG2，Huh7 / Sor and HepG2 / Sor cells (P ＜0. 05 ) .
Conclusion :AMD3100 can reduce the resistance of human hepatocellular carcinoma cells to Sorafenib by downregulating the expression of CA9 and CXCR4，and enhance the inhibition of Sorafenib on the proliferation and invasion of human hepatocellular carcinoma cells.
- Full text:2024081617045314664AMD3100下调CA9与...CR4表达逆转索拉菲尼耐药_秦骥伟.pdf
