LC⁃MS/MS simultaneous determination of 22 bile acids in serum , liver , amniotic fluid and placenta of pregnant mice
10.19405/j.cnki.issn1000-1492.2023.08.007
- Author:
Fan Zhao
1
;
Lun Zhang
2
;
Lu Ye
1
;
Jiayi Zhang
1
;
Yun Yu
2
;
Qianqian Huang
2
;
Jianqing Wang
1
Author Information
1. School of Pharmacy, Anhui Medical University, Hefei 230032; Dept of Pharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230012 ; Anhui Public Health Clinical Center, Hefei 230012
2. Dept of Pharmacy, The First Afiliated Hospital of Anhui Medical University, Hefei 230012 ; Anhui Public Health Clinical Center, Hefei 230012
- Publication Type:Journal Article
- Keywords:
bile acids;
high performance liquid chromatography⁃mass spectrometry system;
pregnant mice;
methodology
- From:
Acta Universitatis Medicinalis Anhui
2023;58(8):1287-1292,1299
- CountryChina
- Language:Chinese
-
Abstract:
Objective :High performance liquid chromatography⁃mass spectrometry (LC⁃MS/MS) system was used to accurately determine 22 bile acids in serum , liver, amniotic fluid and placenta of pregnant mice , and a LC⁃MS/ MS method was established for efficient detection and analysis of bile acids in serum , liver, amniotic fluid and placenta of mice.
Methods :Pregnant mice serum , liver, amniotic fluid and placenta samples were processed , with 0. 1% glacial acetic acid in 4 mmol/L ammonium acetate aqueous solution as mobile phase A and pure methanol as
mobile phase B , the flow rate was 0. 4 ml/min , a gradient elution program was used to elute with Phenomenex Gemini 3 μm NX⁃C18 110A ( 100 mm × 2. 0 mm) chromatographic column elution , and mass spectrometry detection system used an electrospray ion source for negative ion multiple reaction monitoring.
Results :The linear relationship of 22 bile acids in the quantitative range was good. The RSD of inter⁃day and intra⁃day precision at low , medium and high concentrations was 0. 5% - 7. 4% , the matrix effect was 88% - 110% , and the extraction recovery was 84% - 108% .
Conclusion : In this experiment , LC⁃MS/MS was established to detect 22 bile acids in serum , liver, amniotic fluid and placenta of pregnant mice. The method not only has high sensitivity and selectivity , but also can stably detect a large number of samples.
- Full text:2024081417053092119LC-MS_MS同时测定2...、肝脏、羊水和胎盘中的应用_赵凡.pdf
