QDPR improves UUO⁃induced renal interstitial fibrosis in rats by regulating Beclin1
10.19405/j.cnki.issn1000-1492.2023.05.017
- Author:
Haiyang Gao
1
;
Hailan He
2
;
Liguo Zhang
1
;
Xi Chen
1
;
Zhiguo Li
2
Author Information
1. Dept of Urology,Afiliated Hospital of North China University of Science and Technology, Tangshan 063210
2. School of Public Health ,North China University of Science and Technology, Tangshan 063210
- Publication Type:Journal Article
- Keywords:
quinone dihydrobiopterin reductase;
Beclin1;
renal interstitial fibrosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):806-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of QDPR on renal interstitial fibrosis and Beclin1 expression of unilateral ureteral obstruction ( UUO) rat model.
Methods:The UUO model was constructed by unilateral ureteral ligation. The fibrosis progression , QDPR and Beclin1 expression of UUO model were evaluated by Masson , COL Ⅰ staining and Western blot techniques. UUO model which was over expression QDPR and its control were constructed by retrograde ureteral infusion of corresponding lentivirus. Infection efficiency and QDPR expression level were further evaluated. The QDPR overexpression on fibrosis progression , and Beclin1 was further analyzed.
Results:Compared with the sham operation group , the collagen fibers level in the UUO model group significantly increased (P <0. 05) , the QDPR expression level was significantly reduced (P < 0. 05) , and the Beclin1 significantly increased (P< 0. 05) . Retrograde injection of lentivirus through the ureter could successfully infect the kidney and increase the expression level of QDPR in the kidney. The overexpression of QDPR in kidney could significantly decrease collagen fibers (P < 0. 05) and Beclin1 (P < 0. 05) expression.
Conclusion:Overexpression of QDPR inhibits the expression level of Beclin1 and progression of fibrosis , suggesting that overexpression of QDPR may relieve renal interstitial fibrosis by inhibiting Beclin1expression.
- Full text:2024081221440584099QDPR通过调控Becli...UO诱导的大鼠肾间质纤维化_高海洋.pdf
