Crisaborole ointment regulates abnormal activation of keratinocytes and relieves psoriasis in mice
10.19405/j.cnki.issn1000-1492.2023.05.006
- Author:
Yuqing Gui
1
;
Caihong Tang
1
;
Jingyu Chen
1
;
Ling Jiang
2
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032
2. Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032 ;The First Afiliated Hospital of University of Science and Technology of China , Hefei 230001
- Publication Type:Journal Article
- Keywords:
psoriasis;
crisaborole;
proliferation;
differentiation;
keratinocytes;
PKA;
CREB
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):735-741
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the effect of crisaborole on imiquimod (IMQ) Ⅳinduced psoriasis in mice.
Methods:Forty eight Balb/c mice were randomly divided into crisaborole group (7. 5 , 15 , 30 mg/cm2），halometasone group ( 15 mg/cm2 ) , model group and normal group. IMQ was applied to the back of mice to establish the psoriasis model. Psoriasis area and severity index ( PASI) score was calculated , pathological changes , skin epidermal thickness and inflammatory cell infiltration in the dermis were observed by HE staining. The expressions of keratin (K) 1 , K10 , K6 , K16 and K17 in skin lesions were detected by Western blot and immunohistochemistry. The levels of cyclic adenosine monophosphate ( cAMP) , protein kinase A ( PKA) and phospho⁃cAMP response element binding protein (p⁃CREB) were detected.
Results :Compared with the model group , the PASI score of the crisaborole group decreased , the expression levels of proliferative keratin ( K6 , K16 and K17 ) decreased( F = 12. 62、19. 41、28. 39 ,P < 0. 01) , and the expression levels of differentiation keratin (K1 and K10) increased(F = 27. 95、9. 64 , P < 0. 01) .
Conclusion:Crisaborole plays a therapeutic role in IMQ⁃induced psoriasis in mice by regulating the abnormal proliferation and differentiation of keratinocytes.
- Full text:2024081022502289688克立硼罗软膏调节角质形成细...异常活化缓解小鼠银屑病作用_桂雨晴.pdf
