Simultaneous determination of four diterpene lactones in Lianzhi Xiaoyan capsules by QAMS

VernacularTitle:一测多评法测定莲芝消炎胶囊中4种二萜内酯
Author: BI Fujun ; LU Qiaoyin ; LIN Tong
Publication Type:Journal Article
Keywords: Lianzhi Xiaoyan capsules; andrographolide; diterpene lactone; correction factor; QAMS; internal standard method
From: Drug Standards of China 2024;25(1):048-054
CountryChina
Language:Chinese
Abstract: Objective: To set up a multi-components by single maker (QAMS) method for determination of four diterpene lactones in Lianzhi Xiaoyan capsules.
Methods: Shimadzu Shim-pack C₁₈ column（250 mm×4.6 mm,5 μm）was adopted with 35 ℃. Acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase in gradient mode was used at flow rate of 1.0 mL·min^-1. The detection wavelength was set at 203 nm. Andrographolide was used as reference to calculate the relative correction factors of the other three components.
Results: The linear relationships of four components were good （r≥0.999 9） within their ranges, and their average recoveries were 100.0%-102.8% with RSDs 1.1%-2.2%. The results obtained by QAMS were close to those obtained by external standard method.
Conclusion: This rapid, precise and reproducible method can be used for the quality control of Lianzhi Xiaoyan capsules.
Full text:202408101322012651320240208.pdf