Accuracy of capillary blood 3-beta-hydroxybutyrate determination for the detection and treatment of canine diabetic ketoacidosis.
10.4142/jvs.2014.15.2.309
- Author:
Francesca BRESCIANI
1
;
Marco PIETRA
;
Sara CORRADINI
;
Massimo GIUNTI
;
Federico FRACASSI
Author Information
1. Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy. federico.fracassi@unibo.it
- Publication Type:Original Article
- Keywords:
acetoacetate;
diabetes mellitus;
dog;
ketone bodies
- MeSH:
3-Hydroxybutyric Acid/blood/*diagnostic use;
Animals;
Blood Chemical Analysis/standards/*veterinary;
Blood Specimen Collection/instrumentation/*veterinary;
Capillaries/chemistry;
Diabetic Ketoacidosis/diagnosis/therapy/*veterinary;
Dog Diseases/*diagnosis/therapy;
Dogs;
Electrochemical Techniques/instrumentation/*veterinary
- From:Journal of Veterinary Science
2014;15(2):309-316
- CountryRepublic of Korea
- Language:English
-
Abstract:
In human medicine, diagnosis of diabetic ketoacidosis (DKA) is usually based on measurement of capillary 3-beta-hydroxybutyrate (3-HB) with a hand held ketone sensor. This study was conducted to determine if measurement of capillary 3-HB could be useful for the diagnosis and monitoring of canine DKA. Fifteen dogs with diabetic ketosis and 10 with DKA were evaluated. Paired measurements of 3-HB of capillary and venous blood samples were analysed by the electrochemical sensor and reference method. Use of capillary 3-HB measurement during DKA management was then evaluated through simultaneous measurements of capillary 3-HB, urinary AcAc and venous blood gas analysis. Good agreement between capillary and venous 3-HB measurement was detected by the electrochemical sensor and reference method. Monitoring treatment of DKA revealed a significant correlation between capillary 3-HB and acidosis markers, while no significant correlation was observed between AcAc and acidosis markers. A cut-off value of capillary blood 3-HB >3.8 mmol/L for diagnosis of DKA resulted in 70% and 92% sensitivity and specificity. The electrochemical sensor accurately measures 3-HB concentration in both capillary and venous blood samples, is accurate in diagnosing canine DKA, and appears to reflect the patient's metabolic status during DKA treatment.
