The preventive effect of Gastrodia elata Blume extract on vancomycin‑induced acute kidney injury in rats
10.1186/s42826-024-00200-y
- Author:
Yeon Su LEE
1
;
Yu Rim PARK
;
Hyo Bin LEE
;
Hye Joon PARK
;
Ha Eun LEE
;
Geon A KIM
;
Sang‑Hoon KIM
;
Jae‑Ho SHIN
Author Information
1. Department of Senior Healthcare, Eulji University, Uijeongbu 11549, Korea
- Publication Type:RESEARCH
- From:Laboratory Animal Research
2024;40(2):169-180
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Gastrodia elata Blume (GEB), a traditional medicinal herb, has been reported to have pharmacological effect including protection against liver, neuron and kidney toxicity. However, explanation of its underlying mecha‑ nisms remains a great challenge. This study investigated the protective effects of GEB extract on vancomycin (VAN)-induced nephrotoxicity in rats and underlying mechanisms with emphasis on the anti-oxidative stress, anti-inflam‑ mation and anti-apoptosis. The male Sprague-Dawley rats were randomly divided three groups: control (CON) group, VAN group and GEB group with duration of 14 days.
Results:The kidney weight and the serum levels of blood urea nitrogen and creatinine in the GEB group were lower than the VAN group. Histological analysis using hematoxylin & eosin and periodic acid Schiff staining revealed pathological changes of the VAN group. Immunohistochemical analysis revealed that the expression levels of N-acetyl-D-glucosaminidase, myeloperoxidase and tumor necrosis factor-alpha in the GEB group were decreased when compared with the VAN group. The number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells, phosphohistone and malondialdehyde levels were lower in the GEB group than VAN group.The levels of total glutathione in the GEB group were higher than the VAN group.
Conclusions:The findings of this study suggested that GEB extract prevents VAN-induced renal tissue damage through anti-oxidation, anti-inflammation and anti-apoptosis.
