Pulmonary passage of canine Regenerative Medicine adipose tissue-derived mesenchymal stem cells through intravenous transplantation in mouse model
- Author:
Jaeyeon KWON
1
;
Mu-Young KIM
;
Soojung LEE
;
Jeongik LEE
;
Hun-Young YOON
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(3):e36-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:The present study assessed the occurrence of PTE after the intravenous infusion of canine AdMSCs (cAdMSCs) into experimental animals.
Methods:Five-week-old male BALB/c hairless mice were categorized into groups labeled A to G. In the control group (A), fluorescently stained 2 × 106 cAdMSCs were diluted in 200 μL of suspension and injected into the tail vein as a single bolus. The remaining groups included the following: group B with 5 × 106 cells, group C with 3 × 106 cells, group D with 1 × 106 cells, group E with 1 × 106 cells injected twice with a one-day interval, group F with 2 × 106 cells in 100 μL of suspension, and group G with 2 × 106 cells in 300 μL of suspension.
Results:Group D achieved a 100% survival rate, while none of the subjects in groups B and C survived (p = 0.002). Blood tests revealed a tendency for the D-dimer levels to increase as the cell dose increased (p = 0.006). The platelet count was higher in the low cell concentration groups and lower in the high cell concentration groups (p = 0.028). A histological examination revealed PTE in most deceased subjects (96.30%).
Conclusions:and Relevance: PTE was verified, and various variables were identified as potential contributing factors, including the cell dose, injection frequency, and suspension volume.
