Isolation and characterization of feline endometrial mesenchymal stem cells
- Author:
Mi-Kyung PARK
1
;
Kun-Ho SONG
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2024;25(2):e31-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Recently, there has been a growing interest in stem cells for human medicine.Limited feline endometrial mesenchymal stem cell (fEM-MSC) research in veterinary medicine necessitates reporting for future feline disease research and therapy.
Objectives:This study aimed to isolate fEM-MSCs from feline endometrial tissues and evaluate their morphology, proliferative ability, differentiation ability, and immunophenotype.
Methods:Feline endometrial tissues were obtained from the ovariohysterectomies of healthy cats and isolated using an enzymatic method. The morphology and proliferative ability of the isolated cells were assessed using a doubling time (DT) assay from passages 3 to 6 (P3 − P6). We measured pluripotency gene expressions of cells in P2 using quantitative realtime polymerase chain reaction (qRT-PCR). To investigate MSC characteristics, a trilineage differentiation assay was conducted in P4, and cells in P4 were immunophenotyped using flow cytometry.
Results:fEM-MSCs showed a typical spindle-shaped morphology under a microscope, and the DT was maintained from P3 to P6. fEM-MSCs could differentiate into adipocytes, osteoblasts, and chondrocytes, and expressed three pluripotency markers (OCT4, SOX2, and NANOG) by qRT-PCR. Immunophenotypic analysis showed that the fEM-MSCs were CD14− , CD34− , CD45− , CD9+ , and CD44+ .
Conclusions:In this study, the feline endometrium was a novel source of MSCs, and to the best of our knowledge, this is the first report on the isolation method and characteristics of fEM-MSCs.
