Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue
- Author:
Syeo Young WEE
1
;
Jeong Do PARK
;
Sang Gue KANG
;
Eun Soo PARK
Author Information
- Publication Type:Original Article
- From:Archives of Aesthetic Plastic Surgery 2024;30(1):16-21
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Adipose tissue-derived stem cells (ASCs) represent a rapidly evolving area of cell-based therapies. ASCs are harvested from the stromal vascular fraction (SVF), a heterogeneous mixture of ASCs and other cell types. Two methods are typically employed to obtain the SVF: enzymatic and mechanical. In this study, we examined the efficacy of ultrasonic cavitation in isolating the SVF from adipose tissue.
Methods:Human adipose tissue was procured from three patients through an aesthetic liposuction procedure. This tissue was then subjected to either an enzymatic method utilizing type II collagenase or an ultrasonic cavitation method using an Ultra Stemcell device. The cell counts and viability were determined using a cell counter. The immunophenotype of the SVF was analyzed using real-time polymerase chain reaction.
Results:Compared to the enzymatic method, the total cell count and cell viability of the SVF isolated through ultrasonic cavitation were relatively low. However, no significant difference was observed in the immunophenotype of CD45, CD11b, CD34, and CD105.
Conclusions:The ultrasonic cavitation method constitutes a clinically practical approach that offers straightforward access in the operative field. This technique is a rapid and cost-effective method for isolating the SVF.
