The role of FAM134B-mediated autophagy in endoplasmic reticulum stress and hippocampal neurons apoptosis induced by Mg2+ -free solution
10.19845/j.cnki.zfysjjbzz.2020.0384
- VernacularTitle: FAM134B介导的自噬在无镁外液致痫海马神经元内质网应激及凋亡中的作用
- Author:
Nanchang XIE
1
;
Yujuan LI
1
;
Yingjiao LI
1
Author Information
1. Department of Neurology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China
- Publication Type:Journal Article
- Keywords:
FAM134B;
Autophagy;
Epilepsy;
Endoplasmic reticulum stress
- From:
Journal of Apoplexy and Nervous Diseases
2020;37(6):519-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of FAM134B-mediated autophagy in endoplasmic reticulum stress and hippocampal neurons apoptosis induced by Mg2+-free solution.Methods Hippocampal neurons from newborn SD rats were randomly divided into control group,AE group,Lenti-pGV group,Lenti-FAM134B group and Lenti-FAM134B-shRNA group.In addition,the selectively autophagy inhibitor (3-Methyladenine,3-MA) was used.LDH assay was used to assess hippocampal neuron activity.TUNEL assay was used to measure apoptotic neurons.The expression of GRP78,CHOP and LC3-Ⅱ/LC3-Ⅰ were detected by Western blotting.Results Compared with the control group,the release rate of LDH was increased,the hippocampal neuron activity was decreased,and neuronal apoptosis was significantly increased in AE group,while FAM134B Overexpression decreased the release rate of LDH,increased the hippocampal neuron activity and attenuated AE-induced neuronal apoptosis;the ratio of LC3-Ⅱ/LC3-Ⅰ was significantly increased in the AE groups,while FAM134B Overexpression further increased the ratio of LC3-Ⅱ/LC3-Ⅰ;the expression of GRP78 and CHOP were obviously increased in the AE groups,while FAM134B Overexpression decreased the expression of GRP78 and CHOP induced by AE;in addition,FAM134B down-expression all exerted the opposite effect.Compared with Lenti-FAM134B group,3-MA abolished the effects of FAM134B Overexpression on the expression of GRP78 and CHOP and the hippocampal neuron activity.In addition,3-MA significantly increased the expression of GRP78 and CHOP and the release rate of LDH.Conclusion FAM134B-mediated autophagy may play a protective role in endoplasmic reticulum stress and hippocampal neurons apoptosis induced by Mg2+-free solution.
- Full text:2024080122423738836The role of FAM134B-mediated autophagy in endoplasmic reticulum stress and hippocampal neurons apoptosis induced by Mg2+ -free solution.pdf
