Effect of Human Urinary Kallindinogenase on cerebral ischemia/reperfusion injury through downregulating NLRP3 inflammasome in mice
- VernacularTitle:人尿激肽原酶通过调控NLRP3炎性小体对小鼠脑缺血/再灌注损伤的影响
- Author:
Dong HAN
1
;
Lulu WEN
1
;
Jue WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Human Urinary Kallindinogenase; Cerebral ischemia/reperfusion; NLRP3 inflammasome
- From: Journal of Apoplexy and Nervous Diseases 2020;37(7):629-632
- CountryChina
- Language:Chinese
- Abstract: Objective To study the protective effect and possible mechanism of Human Urinary Kallindinogenase （UK） after ischemia-reperfusion injury in mice. Methods Middle cerebral artery occlusion （MCAO） was establish for 1 h and then reperfusion to mimic ischemic stroke in mice. UK treatment with high （35×10 -3 PNAU/kg） and low （17.5×10 -3 PNAU/kg） does were intravenous injection after reperfusion for 30 min，respectively. 2，3，5-triphenyltetrazolium chloride （TTC） staining was used to assay cerebral infarction size，neurological assessment was performed according to Bederson method. Elisa methods was applied to determine serum and brain tissue IL-1β、IL-18 content，and Real time PCR and Western blot were exerted to evaluate NLRP3，ASC，and caspase-1 mRNA and protein expression. Results The results showed that UK treatment attenuated infarction size in ischemic stroke mice，and improve neurological impairment. Compared with sham mice，NLRP3，ASC，and caspase-1 mRNA and protein expression were elevated，IL-1β and IL-18 concentration were increased obviously in MCAO/R mice. Human Urinary Kallindinogenase treatment inhibited IL-1β and IL-18 releasing，downregulating NLRP3，ASC，and caspase-1 mRNA and protein expression. Conclusion UK attenuates inflammatory response and infarction size partly through inhibiting NLRP3 inflammasome expression and cytokine releasing.
- Full text:202408012203178845Effect of Human Urinary Kallindinogenase on cerebral ischemia_reperfusion injury through downregulating NLRP3 inflammasome in mice.pdf
