The role of miR-132 in primary cortical neuron ischemic injury induced by oxygen-glucose-deprivation
- VernacularTitle:miR-132在氧糖剥夺诱导原代皮质神经元缺血损伤中的作用
- Author:
Liqiang YANG
1
;
Weijie XU
1
;
Xin GUAN
1
Author Information
- Publication Type:Journal Article
- Keywords: MiR-132; Oxygen-glucose-deprivation; Ischemic injury; Primary cortical neurons
- From: Journal of Apoplexy and Nervous Diseases 2020;37(8):676-679
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the role of miR-132 in oxygen-glucose-deprivation （OGD） induced ischemic injury model using primary cortical neurons.Methods Cultured primary cortical neurons were exposed to oxygen-glucose-deprivation （OGD）for 2 h followed by 24 h reperfusion.The cells were randomly divided into sham operation group (Sham),OGD group,lentivirus control group (LV-control),miR-132 low expression group (LV-anti-miR-132),and miR-132 overexpression group (LV-miR-132).CCK-8,real-time PCR,and Western blot were used to study the effects and mechanisms of miR-132 on primary cortical neurons injured by OGD.Results The expression levels of miR-132 were significantly reduced in the OGD-induced ischemic injury model (P<0.05).The reduced expression of miR-132 aggravated OGD injury and significantly reduced cell viability in OGD-induced primary cortical neuron ischemic injury.On the contrary,the increased expression levels of miR-132 reduced OGD injury and increased cell survival.Western blot results showed that overexpression of miR-132 upregulated the levels of synapsin-1 and PSD95.Conclusion Overexpression of miR-132 can improve OGD-induced ischemic injury and increase the survival of primary cortical neurons by upregulating the synapsin-1 and PSD95.
- Full text:2024073122324489316The role of miR-132 in primary cortical neuron ischemic injury induced by oxygen-glucose-deprivation.pdf
