Effect of wogonoside on KLF4/NF⁃κB signal pathway in RAW264. 7 macrophages stimulated by high glucose
10.19405/j.cnki.issn1000-1492.2022.10.003
- Author:
Shan Xie
1
;
Xingxin Xu
1
Author Information
1. Dept of Nephrology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei 230001;Dept of Nephrology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
macrophages;
wogonoside;
KLF4
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1523-1528
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effect of wogonoside(WG)on the Krüppel ⁃like factor4(KLF4)/nuclear factor κB(NF⁃κB) pathway of RAW264. 7 macrophages subject to high glucose stimulation.
Methods :RAW264. 7 macrophages were cultured and the concentration range of inactive effect of WG on cells was detected by CCK⁃8. Macrophages randomly grouped into LG group (medium for 5. 5 mmol/L of glucose), D group (30 mmol/L mannitol medium), HG group ( medium for 30 mmol/L glucose), LG + WG50 group (5. 5 mmol/L glucose + 50 μmol/L WG), HG + WG12. 5 group (30 mmol/L glucose + 12. 5 μmol/L WG), HG + WG25 group (30 mmol/L glucose + 25 μmol/L WG), HG + WG50 group (30 mmol/L glucose + 50 μmol/L WG) . The expression of inducible nitric oxide synthase( iNOS) in cells of each group was determined by immunofluorescence. NF⁃κB p65, NF⁃κB pp65, KLF4, iNOS, interleukin⁃1β(IL⁃1β), tumor necrosis factor alpha( TNF⁃α) expression in each group was detected by Western blot. mRNA expression of KLF4, iNOS,IL⁃1β, TNF⁃α in each group was detected by qRT⁃PCR. IL⁃1β, TNF⁃α secretion of cells in each group was detected by ELISA.
Results :Compared with LG group,the expression of iNOS in HG group was higher(P < 0. 01), and the expression of iNOS in HG + W50 group was lower than that in HG group(P < 0. 01) . Compared with the LG group, high glucose can inhibit KLF4 expression (P < 0. 01), stimulate NF⁃κB pp65, iNOS,IL⁃1β, TNF⁃α expression(P < 0. 01), and increase the IL⁃1β, TNF⁃α level in the supernatant(P < 0. 01); However, the inflammatory index in the WG groups significantly decreased (P < 0. 01), but expression of KLF4 dose⁃dependent increased (P < 0. 01) .
Conclusion :WG inhibits the expression of inflammatory factors and associated proteins secreted by macrophages by high glucose, and has the therapeutic effect of improving the protective factor⁃KLF4.
- Full text:2024073121453649628汉黄芩苷干预高糖刺激RAW...LF4_NF-κB信号通路_谢姗.pdf
