LINC00612 targeted Bcl-2 to inhibit the apoptosis of neurons incubated with Aβ1-42
- VernacularTitle: LINC00612靶向结合Bcl-2抑制Aβ1-42孵育的神经元凋亡
- Author:
Qian PENG
1
;
Defeng ZHAO
1
;
Jian ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: LINC00612 ； Bcl-2 ； Aβ1-42 ； SH-SY5Y cells
- From: Journal of Apoplexy and Nervous Diseases 2021;38(9):810-812
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of LINC00612 on apoptosis of SH-SY5Y cells incubated with Aβ1-42.Methods Neuronal injury model of Alzheimer’s disease were established by SH-SY5Y cells incubated with Aβ1-42.The expression of LINC00612 in SH-SY5Y cells incubated with Aβ1-42 was detected by qRTPCR. The LINC00612 overexpressed plasmid was transfected into SH-SY5Y cells incubated with Aβ1-42，and the expression of Bcl-2 was detected by Western blot. Apoptosis was detected by Annexin V-FITC /PI staining. RNA-pulldown and RIP experiments were performed to detect the binding effect of LINC00612 and Bcl-2.Results LINC00612 was low expressed in SH-SY5Y cells incubated with Aβ1-42.Overexpression of LINC00612 significantly increased Bcl-2 expression and decreased apoptosis. RNA pulldown and RIP results showed that LINC00612 could bind to Bcl-2.Conclusion Overexpression of LINC00612 can up-regulate Bcl-2 expression and inhibit the apoptosis of SH-SY5Y cells incubated with Aβ1-42.
- Full text:2024072910395403841LINC00612 targeted Bcl-2 to inhibit the apoptosis of neurons incubated with Aβ1-42.pdf
