Therapeutic effect of isoliquiritigenin on rat hepatic fibrosis and its mechanism
10.19405/j.cnki.issn1000-1492.2023.04.021
- Author:
Qi Zhang
1
;
Zhenxiang An
2
;
Yuanli He
3
Author Information
1. The First Clinical Medical College of Guizhou University of Traditional Chinese Medicine，Guiyang 550002
2. The First Clinical Medical College of Guizhou University of Traditional Chinese Medicine，Guiyang 550002;Dept of Gastroenterology，The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550001
3. Dept of Geriatrics，The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine，Guiyang 550001
- Publication Type:Journal Article
- Keywords:
hepatic fibrosis;
HIF-1 α;
VEGF;
isoliquiritigenin
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):649-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the therapeutic effect and mechanism of isoliquiritigenin (ISL) on hepatic fibrosis (HF) ．
Methods :SD rats were randomly divided into normal group，model group，colchicine tablet group (positive control，0. 1 mg / kg) ，and high-dose and low-dose ISL groups (40 ，10 mg / kg) ．Except for the normal group，the other groups were given intraperitoneal injection of 50% carbon tetrachloride ( CCl4 ) olive oil solution ( 1. 5 ml / kg) to establish liver fibrosis models，twice a week for 8 weeks．After modeling，rats in each group were given the corresponding drugs by gavage at a volume of 10 ml / kg per day for 4 weeks．After the last administration， the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum of rats were determined ; liver index was calculated ; HE and Masson staining were used to observe the pathological changes of liver tissue ; Real-time quantitative PCR was used to detect the mRNA expression levels of hypoxia-inducible factor-1α (HIF-1 α) and vascular endothelial growth factor ( VEGF) ; the expressions of HIF-1α and VEGF protein in liver tissue were detected by immunohistochemistry and Western blot.
Results:Compared with the normal group，the serum ALT and AST contents of the rats in the model group increased (P＜0. 01) ，the liver index increased (P < 0. 01) ，the rat fibrous tissue hyperplasia，the collagen volume fraction increased (P＜0. 01) ，and the liver the expression levels of HIF-1 α and VEGF mRNA in the tissue increased (P＜0. 01) ．Immunohistochemistry and West- ern blot showed that the expression levels of HIF-1α and VEGF protein increased in the model group (P＜0. 01) . Compared with the model group，the ISL high and low dose groups could reduce the levels of ALT and AST in serum (P＜0. 01) ，the liver index (P＜0. 01) ，the proliferation of fibrotic tissue and the collagen volume fraction (P ＜0. 05) ，down-regulate the expression levels of HIF-1 α and VEGF genes (P＜0. 01) ．Immunohistochemical detection showed that the high and low dose groups of ISL could reduce the protein expression levels of HIF-1α (P < 0. 01，P＜0. 05) ，the expression level of VEGF protein decreased (P＜0. 01) ，Western blot detection showed that the high-dose group of ISL could reduce the protein expression levels of HIF-1α and VEGF (P＜0. 05) .
Conclusion :ISL has a significant therapeutic effect on CCl4 -induced HF model rats，and its mechanism may be related to the regulation of HIF-1α and VEGF protein expression levels.
- Full text:2024072719355674326异甘草素对大鼠肝纤维化的治疗作用及其机制_张畦.pdf
