Effects of Blonanserin on hippocampal neurons damage in schizophreniarats by activating PI3K /AKT / GSK3 β signaling pathways
10.19405/j.cnki.issn1000-1492.2022.11.020
- Author:
Xiaojin Xu
1
;
Maosheng Fang
1
;
Ying Miao
1
;
Weiyong Li
2
Author Information
1. Dept of Psychiatry，Wuhan Mental Health Center，Wuhan 430012
2. Dept of Pharmacy， Union Hospital of Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022
- Publication Type:Journal Article
- Keywords:
Blonanserin;
schizophrenia;
PI3K / AKT / GSK3 β signaling pathway;
cognitive function;
learning and memory;
glucose-lipid metabolism
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1801-1806
- CountryChina
- Language:Chamorro
-
Abstract:
Objective :To explore the effects of Blonanserin on hippocampal neurons damage and PI3K / AKT / GSK3 β signaling pathways in rats with MK-801 induced schizophrenia．
Methods :The schizophrenia models were induced by one-time intraperitoneal injection of MK-801．A total of 48 SD rats were randomly divided into normal group，model group，Blonanserin group ( 1 mg / kg Blonanserin) and risperidone group (0. 54 mg / kg risperidone) ， with 12 cases in each group．The behaviors of rats were observed by stereotyped behavior assay，open field test and Morris water maze．The pathological damage of hippocampus was observed by HE staining，which was scored．The levels of serum glucose-lipid metabolism indexes were detected．The mRNA and proteins of phosphatidylinositol 3- kinase (PI3K) ，protein kinase B (AKT) and glycogen synthase kinase 3 β ( GSK3 β) in hippocampal tissues were detected by real-time fluorescent quantitative PCR and Western blot.
Results :Compared with model group，scores of stereotyped behaviors，total distance in open field test，escape latency and score of pathological damage in hippo- campus decreased in Blonanserin group and risperidone group，while times of crossing platform original site，levels of PI3K，AKT and GSK3 β mRNA，and phosphorylation levels of PI3K，AKT and GSK3 increased (P ＜0. 05) . However，there was no significant difference in the above indexes between Blonanserin group and risperidone group．The differences in levels of fasting blood glucose ( FPG) ，glycosylated hemoglobin ( HbA1c) ，triglyceride (TG) ，total cholesterol (TC) ，high-density lipoprotein (HDL) and low-density lipoprotein (LDL) among normal group，model group and Blonanserin group was not statistically significant．However，levels of FPG，HbA1c，TG and TC in risperidone group were higher than those in the other three groups (P＜0. 05) ，and there was no signifi- cant change in HDL or LDL．
Conclusion :Blonanserin may protect schizophrenia rats from hippocampal neurons damage，improve cognitive function，learning and memory ability by activating PI3K / AKT / GSK3 β signaling path- ways.
- Full text:2024072521301126172布南色林通过激活PI3K_...症大鼠海马神经元损伤的影响_徐晓津.pdf
